The Journal of Neuroscience, December 27, 2006, ():
Synaptotagmin-2 Is Essential for Survival and Contributes to Ca2+ Triggering of Neurotransmitter Release in Central and Neuromuscular Synapses
J. Neurosci. Pang et al.
26: 13493
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Suppl. Figure_1. A, Southern blot and B, PCR reaction for detection of synaptotagmin-2 mutant allele.
- supplemental material
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Suppl. Figure_2. Whole-mount diaphragm immunohistochemistry. A, Synaptotagmin-2 and α-bungarotoxin staining of NMJ in whole mount diaphragm staining in wildtype (WT), and B, synaptotagmin 2 KO. Bar=100 μm.
- supplemental material
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Suppl. Movie_1. Video of synaptotagmin-2 KO and their littermate wildtype and heterozygous controls at postnatal day 15. Motor function of synaptotagmin-2 KO animals is severely impaired, such that they can hardly move. Their littermate controls are moving normally.
- supplemental material
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Suppl. Movie_2. Three-D reconstruction of a NMJ in wildtype after immunohistochemistry using antibody against synaptotagmin-2 (A320) and Alexa 594-conjugated α-bungarotoxin to label nicotinic acetylcholine receptors.
- supplemental material
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Suppl. Movie 3. Three-D reconstruction of a NMJ in synaptotagmin-2 KO after immunohistochemistry using antibody against synaptotagmin-2 (A320) and Alexa 594-conjugated α-bungarotoxin to label nicotinic acetylcholine receptors.
