Figure 3.
Expression pattern of synaptotagmin-2 in forebrain. β-Galactosidase activity is revealed by X-gal staining in synaptotagmin-2 KO homozygous mice. A, The majority of striatal neurons show β-galactosidase activity. B, C, Higher magnifications of brain areas indicated by dashed squares in A. D, No synaptotagmin-2 expression is found in olfactory bulb. E, Scattered neurons in retina showing β-galactosidase positivity. F, Very few neurons in cortex and hippocampus are β-galactosidase positive. G–J, Higher magnifications of brain areas indicated by dashed squares in F. 3V, Third ventricle; Cg, cingulate cortex; Ctx, cortex; CPu, caudate–putamen (striatum); DG, dentate gyrus; EPl, external plexiform layer of olfactory bulb; G, glomeruli; GC, ganglion cell layer; GrO, granular cell layer of olfactory bulb; INL, inner nuclear layer; LGP, lateral globus pallidus; LV, lateral ventricle; Mi, mitral cell layer of olfactory bulb; PEp, pigment epithelium; SO, stratum oriens; SR, stratum radiatum; R & C, layer of rods and cones; Rt, reticular thalamic nucleus; VMH, ventromedial hypothalamic nucleus; ZI, zona incerta. Scale bars: A, F, 50 μm; B–D, G–J, 5 μm; E, 2.5 μm.