The Journal of Neuroscience, February 8, 2006, ():
MTH1, an Oxidized Purine Nucleoside Triphosphatase, Suppresses the Accumulation of Oxidative Damage of Nucleic Acids in the Hippocampal Microglia during Kainate-Induced Excitotoxicity
J. Neurosci. Kajitani et al.
26: 1688
Supplemental data
Files in this Data Supplement:
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Suppplemental methods
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Supplemental Figure 1. Histological damage in the brain after systemic kainite administration. Male C57BL/6J mice were injected with 30 mg/kg of kainate or saline (control), and then were sacrificed at 72 h and 1 week after the injection. The brain sections prepared were stained with hematoxylin and eosin (HE) (a-c) or subjected to immunohistochemistry with anti-GFAP polyclonal antibodies (GFAP) (d-f). No obvious alteration was observed 72 h after kainate administration, however, evident neurodegeneration and astrogliosis were observed at 1 week after kainate administration in the CA3 subregion. Scale bar: 100 µm.
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Supplemental Figure 2. Specific immunodetection of 8-oxoguanine in the normal mouse brain. (A) Detection of 8-oxoG in DNA of the CA3 subregion. The sections prepared from C57BL/6j mice were subjected to immunohistochemistry with the N45.1 mAb after RNase and HCl treatment (a, b). The section pretreated with DNase (c) exhibited little reactivity with the antibody, and pre-adsorption of the antibody with 8-oxo-dG (e) but not with dG (d) abolished the immunoreactivity. (B) Detection of 8-oxoG in RNA of the CA3 subregion. The sections were subjected to immunohistochemistry with the 15A3 mAb without pre-treatment. Cytoplasmic immunoreactivities were mostly observed in the neurons of the hippocampus (a, b). The section pretreated with RNase (c) exhibited little reactivity with the antibody, and pre-adsorption of the antibody with 8-oxo-dG (e) but not with dG (d) abolished the immunoreactivity. Scale bars: 500 µm (a), 100 µm (b-e).
