The Journal of Neuroscience, February 8, 2006, ():
Impaired Neuronal Positioning and Dendritogenesis in the Neocortex after Cell-Autonomous Dab1 Suppression
J. Neurosci. Olson et al.
26: 1767
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Fig. 1.
Abnormal branching patterns of cells in scrambler explants.
(A-E) Sections from explants labeled with sindbis virus (green), immunostained with the nuclear dye Hoechst (blue) and Reelin (red: mAb CR50) reveal morphological differences in migrating neurons depending on genotype. In control explants (+/ ) the majority of GFP+ cells in the CP extended processes into the Reelin expression domain (RED) and the majority of these processes are branched. The yellow arrowheads indicate the extent of the leading process and the white arrowhead shows the location of the branch point if present. (F) Schematic of cell categories used in quantification. (G) Bar chart comparing cellular categories from control (+/ ) and scrambler (-/-) explants and showing cells in each category as a percentage of the total number counted. (H) More cells in in the scrambler (-/-) explants do not contact Reelin expression domain compared to control (+/ ) (*=p=0.03, Student's T-test, single tailed, DF=4). Labeled cells in 6 brains from 2 litters were analyzed: n=68 cells in 3 -/- brains, n=75 cells in 3 control (+/ ) brains. Scale bar is 25 μm.
- supplemental material
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Supplemental Fig. 2.
Model of migration abnormalities in normal and Reelin deficient cortical development. During normal cortical development migrating neurons extend a leading process through the cell dense region of the upper cortex (CP) into the MZ. Reelin signaling leads to the formation of a branched leading process. The cell body translocates up the leading process to the branch point where it arrests. In the absence of Reelin signaling, branch points form improperly or not at all and (D) early born neurons (layer 6) cells translocate imprecisely into the preplate causing a failure of preplate splitting. Later born (layer 2/3) fail to form stabilized branches and arrest in deep positions underneath cell dense cortical plate regions or in some cases, underneath ectopic thalamocortical fascicles (see Tabata and Nakajima, 2002). Later born neurons arrest under cell dense layers of earlier born cells leading to the approximate inversion of layers observed in the absence of Reelin signaling.
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