The Journal of Neuroscience, February 8, 2006, ():
Telencephalin Slows Spine Maturation
J. Neurosci. Matsuno et al.
26: 1776
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Surface expression of TLCN on dendritic filopodia and shaft. Firstly, TLCN on the plasma membrane of living hippocampal neurons was immunolabeled with guinea pig anti-TLCN/Fc antibody (recognizing the extracellular region of TLCN) in the culture medium for 1 hr. After fixation, the neurons were permeabilized with 0.25% Triton X-100 and then incubated with rabbit anti-TLCN antibody (recognizing the cytoplasmic tail of TLCN). (A) Surface labeling. (B) Intracellular labeling. (C) Merged image. The labeling pattern of surface TLCN on dendritic filopodia and shaft (A) is almost same as that of total TLCN (B). Scale bar, 5 μm.
- supplemental material
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Supplemental Figure 2. TLCN slows spine maturation. TLCN on dendritic membrane may have an essential role as a negative regulator for spine maturation through the preservation of dendritic filopodia and small head spines. Because dendritic filopodia and small head spines are more dynamic structures rather than mature spines, TLCN may facilitate flexibility of synapses in contrast to other cell recognition/adhesion molecules promoting stabilization of synapses. The balance of bidirectional forces for structural maturation of spines would be an essential mechanism to regulate the capacity for plasticity during developmental synaptogenesis and subsequent experience-dependent refinement.
