The Journal of Neuroscience, February 15, 2006, ():
Essential Role of the Main Olfactory System in Social Recognition of Major Histocompatibility Complex Peptide Ligands
J. Neurosci. Spehr et al.
26: 1961
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Movie 1 The preference test employed in this study (“cotton-tip-test”) was designed to give mice full access to investigate cotton tipped applicators treated with a given stimulus. The example shown here demonstrates that, during an investigative bout, mice will approach, sniff and invariably come into direct physical contact with the stimulus source.
- supplemental material
-
Figure 7 Cotton-tip-test. Plots of the results from individual mice confirm the averaged group data of the preference tests shown in Fig. 6 and provide an indication of animal variability. Graphs represent percent of time above 50% that each individual male (numbered 1–76, grey bars) spent investigating (A) same-strain urine (left) versus BALB/c urine (right) or (B-G) same-strain urine supplemented with either C57BL/6 peptides (or solvent for F and G) (left) versus BALB/c peptides (right). The total amount of time an individual spent investigating (during the five-minute test period) is listed in the column to the right of the graphs. Group mean values (black bars) are also shown. Statistics were performed on the total amount of time spent investigating each stimulus (for details see Fig. 6).
- supplemental material
-
Figure 8 Cotton-tip-test using decreased peptide concentrations. Group data (A) and individual data (B) showing the preference of male C57BL/6 mice for female same-strain urine supplemented with mixtures of BALB/c (grey) versus C57BL/6 (black) peptides at a peptide concentration of 1.25 µM each (64.5 ± 4.9% vs. 35.5 ± 4.9%; n = 14; P = 0.011; Fig. 8A).
