The Journal of Neuroscience, February 22, 2006, ():
Endogenous Neurogenesis Replaces Oligodendrocytes and Astrocytes after Primate Spinal Cord Injury
J. Neurosci. Yang et al.
26: 2157
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1: Apoptosis and BrdU cell labeling remote from lesion site (lumbar cord). Fragmented nuclei in anterogradely degenerated corticospinal tract in lumbar cord by (a) Nissl stain, 9 weeks post-injury,and (b) Hoechst label, 7 months post-lesion. Arrows indicate fragmented nuclei; arrowheads indicate adjacent intact nuclei. (c) BrdU-labeled cells in the anterogradely degenerated corticospinal tract of the lumbar cord double-label for GFAP, indicating differentiation into astrocytes, and (d) double-label for IBA (arrows), indicating differentiation into microglia 7 months post-lesion. Lumbar tracts containing rostrally-projecting axons, such as the dorsal columns, have few BrdU-labeled cells and most of these express microglial markers (not shown). These findings indicate that newly dividing cells at sites remote from a cervical SCI adopt astrocyte and microglial fates rather than oligodendrocyte fates. Scale bar: a,b, 5μm; c,d, 15μm.
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Supplementary Figure 2: Peri-Lesion Regions From All 4 Subjects.
Nissl stain; * denotes lesion epicenter. Scale bar = 720 μm.
