The Journal of Neuroscience, February 22, 2006, ():
Synaptic Vesicle Recycling Adapts to Chronic Changes in Activity
J. Neurosci. Virmani et al.
26: 2197
Supplemental data
Files in this Data Supplement:
- supplemental material -
Supplementary Figure 1. A-B, Representative traces from electrophysiological experiments performed by bath application of hypertonic sucrose (+500 mOsm) to quantify the size of the readily releasable pool. C, In hippocampal cultures (~20 days in vitro) after TTX or PTX treatments, we detected a decreasing trend in the size of the readily releasable pool determined by integration of the area under the peak current elicited by sucrose. However this decrease, which was especially notable after PTX treatment, did not reach statistical significance (charge transfer during sucrose application, control (n=8): 7.7 ± 1.4 nC, TTX-treated (n=9): 6.5 ± 0.9 nC (p>0.05 vs. control), PTX-treated (n=7): 4.9 ± 0.8 nC, (p=0.06 vs. control)). D, In neocortical cultures (~20 days in vitro), the size of the readily releasable pool of vesicles did not show a significant change after treatment with TTX or PTX (charge transfer during sucrose application, control (n=10): 7.1 ± 0.6 nC, TTX-treated (n=8): 7.2 ± 0.9 nC (p>0.05 vs. control), PTX-treated (n=11): 5.9 ± 0.8 nC, (p>0.05 vs. control)).
- supplemental material -
Supplementary Figure 2. A-C, Representative voltage clamp recording traces from neocortical (top panel) and hippocampal (lower panel) cultures under control (A), after chronic TTX-treated (B) and after chronic PTX treated (C) conditions. D-F, Representative current clamp recording traces from neocortical (top panel) and hippocampal (lower panel) cultures under control (D), after chronic TTX-treated (E) and after chronic PTX treated (F) conditions.
- supplemental material -
Supplementary Figure 3. Representative voltage clamp recording traces depicting the inhibitory and excitatory components of evoked synaptic responses from neocortical (top panel) and hippocampal (lower panel) cultures under control (left), after chronic TTX-treated (middle) and after chronic PTX treated (right) conditions. The inhibitory and excitatory components of evoked synaptic responses were separated pharmacologically.
