The Journal of Neuroscience, January 3, 2007, ():
Hyperexcitability of Distal Dendrites in Hippocampal Pyramidal Cells after Chronic Partial Deafferentation
J. Neurosci. Cai et al.
27: 59
Supplemental Data
Files in this Data Supplement:
- supplemental material
-
Supplemental Fig. 1. Dendritic SK channels contribute to the medium AHP. Whole-cell current-clamp recordings were made from the soma of CA1 cells in hippocampal slice cultures. A patch pipette, containing 1 µM apamin plus Alexa 568 for visualization of the ejection, was positioned in the distal apical dendritic tuft of the cell (image at top left). A train of action potentials was elicited with a 200 ms depolarizing current pulse before and after ejection of the apamin solution in the distal dendrites of the cell (image at top right). Shortly after the ejection of the apamin, there was a broadening of the evoked action potentials and a reduction in the amplitude of the medium AHP (magnified in inset). Pooled data from 9 cells are shown below. The difference in amplitude was significant (p<0.05, paired t-test).
- supplemental material
-
Supplemental Fig. 2. Gradual development of plateau potential prolongation. Plateau potentials were evoked in distal apical dendrites of CA1 cells in control hippocampal slice cultures (white symbols) and sister cultures treated with DNQX (40 µM) and AP5 (80 µM) for 1-6 days (black symbols). We observed a progressive increase in plateau potential duration during this time period. The differences between the two data sets became significant at 3 days (p<0.05, n = 4-9; factorial ANOVA, Tukey post-hoc test).
