The Journal of Neuroscience, March 14, 2007, ():
Natural Oligomers of the Alzheimer Amyloid-ß Protein Induce Reversible Synapse Loss by Modulating an NMDA-Type Glutamate Receptor-Dependent Signaling Pathway
J. Neurosci. Shankar et al.
27: 2866
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Sup. Figure 1. 2-photon laser photoactivation of glutamate
(A) 2PLSM image of a spiny region of apical dendrite of a CA1 hippocampal pyramidal neuron filled with 10 μM Alexa-594 (red fluorescence) and 300 μM of the Ca indicator Fluo-5F. The dashed line shows the orientation of the line scan used in (B)
(B) Red fluorescence measured in a line that intersects the spine head and dendrite shown in (A). A 500 μs pulse of 725 nm laser light directed at the center of the spine head was used to bleach the red fluorescence in the spine. The arrowheads in (A) and (B) show the location and timing, respectively, of the laser pulse
(C) uEPSC (top) and the red fluorescence (bottom) measured using the same laser power indicates that ~ 40% photobleaching results in a ~ 13 pA uEPSC.
(D) Summary of ΔR/R0 measured in the spines used to produce the data shown in Figure 5, showing consistent stimulation across conditions.
(E) uEPSPs (mean ± SEM) measured in control conditions (left) or in the presence of Aβ monomers (middle) or oligomers (right). Differences across conditions are not statistically significant.
