The Journal of Neuroscience, March 21, 2007, ():
Fragile X Mental Retardation Protein Induces Synapse Loss through Acute Postsynaptic Translational Regulation
J. Neurosci. Pfeiffer and Huber
27: 3120
Supplemental Data
Files in this Data Supplement:
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Supplemental Figure 1. Expression Level of FMRP Following Transfection A. Confocal images of FMRP expression levels in wild-type (left) and Fmr1 KO (right) hippocampal slice cultures. The Fmr1 KO slice culture was biolistically transfected with a construct expressing FMRP-GFP driven by the human FMR1 promoter. Both sets of slice cultures were fixed and immuno-labeled with 2F5 FMRP antibody and AlexaFluor568 2° antibody. Scale bar is 10 μm. B. Quantification of somatic FMRP immunofluorescence from confocal images from wild-type and FMRP-transfected Fmr1 KO hippocampal slice cultures. Fluorescence in the KO untransfected neurons was considered background and was subtracted from the WT and KO transfected intensity values. Values are means + SEM. Number of cells quantified is listed on the bar graph. **p < 0.01.
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Table 1
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Table 2
