The Journal of Neuroscience, April 18, 2007, ():
Shape Change Controls Supporting Cell Proliferation in Lesioned Mammalian Balance Epithelium
J. Neurosci. Meyers and Corwin
27: 4313
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplemental Video 1: Wound closure in E18 utricles. Time-lapse images were recorded simultaneously for four utricles every 30 minutes for 24 hours. The supporting cells around the lesion close over the wound and reconstitute epithelial integrity. Time is compressed 18,000x.
- supplemental material
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Supplemental Video 2: Wound closure is blocked by cytochalasin B. Time-lapse images were recorded simultaneously for the three utricles cultured in 1 µM cytochalasin B and the three utricles cultured in 0.1% DMSO, one frame every 30 minutes for 24 hours. While all of the utricles cultured in DMSO have fully closed lesions within the 24 hour record, all of the cytochalasin-treated utricles have open lesions with irregular edges. Following the initial 24 hour recording, the cytochalasin-containing media was washed out and replaced with control media. Time-lapse recording was restarted on the utricles, and revealed normal wound closure in the previously blocked utricles. Time is compressed 18,000x.
- supplemental material
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Supplemental Video 3: Wound closure is inhibited by Y-27632. Time-lapse images were recorded simultaneously for the three utricles cultured in 50 µM Y-27632. The lesions in the Y-27632-treated utricles become regular, smooth, and circular, but do not contract during the initial 24 hour incubation. At the end of the 24 hour recording, Y-27632 was washed out and time-lapse recording restarted. The lesions rapidly close following washout of the inhibitor. Time is compressed 18,000x.
