The Journal of Neuroscience, May 16, 2007, ():
Changes in Synaptic Morphology Accompany Actin Signaling during LTP
J. Neurosci. Chen et al.
27: 5363
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Movie 1. PSD95 and pCofilin immunolabeling are localized to discrete puncta in field CA1. Three dimensional reconstruction of immunolabeling for pCofilin (red) and PSD95 (green) structures (overlap of the two labels appears yellow) generated by restorative deconvolution of wide-field micrographs collected at 0.1 µm z-steps. The movie initially shows a lower power view of the proximal portion of CA1 stratum radiatum (100 x 100 µm), magnifies a portion of this field, and rotates the entire field to reveal differences in the size (volume) of immunoreactive puncta and the incidence of double labeling. For the purpose of illustration, the contrast and the density of the image has been enhanced (this is in distinction from the images collected for quantitative measures: raw images were used for all measures presented in the manuscript).
- supplemental material
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Movie 2. pCofilin-ir puncta exhibit high degree of overlap with PSD95-labeled synapses. Three dimensional image of a small field within CA1 stritum radiatum, showing pCofilin-ir (red, left), PSD95-ir (green, center), and the overlap of the two labels (right, overlap shows yellow) generated by restorative deconvolution of wide-field micrographs collected at 0.1 µm z-steps. Movie shows rotation of the field of labeling at high power and illustrates the differences in the sizes of immunoreactive puncta and the specificity of immunostaining. For the purpose of illustration, the contrast and the density of the image has been enhanced (this is in distinction from the images collected for quantitative measures: raw images were used for all measures presented in the manuscript).
