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The Journal of Neuroscience, May 23, 2007, 27(21):5603-5614; doi:10.1523/JNEUROSCI.4552-06.2007

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Cellular/Molecular
Glial Cell Line-Derived Neurotrophic Factor and Neurturin Inhibit Neurite Outgrowth and Activate RhoA through GFR{alpha}2b, an Alternatively Spliced Isoform of GFR{alpha}2

Li Foong Yoong1 and Heng-Phon Too1,2

1Department of Biochemistry, National University of Singapore, Singapore 119260, and 2Molecular Engineering of Biological and Chemical System/Chemical Pharmaceutical Engineering, Singapore–Massachusetts Institute of Technology Alliance, Singapore 117576


    Abstract
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
The glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) belong to a structurally related family of neurotrophic factors. NTN exerts its effect through a multicomponent receptor system consisting of the GDNF family receptor {alpha}2 (GFR{alpha}2), RET, and/or NCAM (neural cell adhesion molecule). GFR{alpha}2 is alternatively spliced into at least three isoforms (GFR{alpha}2a, GFR{alpha}2b, and GFR{alpha}2c). It is currently unknown whether these isoforms share similar functional and biochemical properties. Using highly specific and sensitive quantitative real-time PCR, these isoforms were found to be expressed at comparable levels in various regions of the human brain. When stimulated with GDNF and NTN, both GFR{alpha}2a and GFR{alpha}2c, but not GFR{alpha}2b, promoted neurite outgrowth in transfected Neuro2A cells. These isoforms showed ligand selectivity in MAPK (mitogen-activated protein kinase) [ERK1/2 (extracellular signal-regulated kinase 1/2)] and Akt signaling. In addition, the GFR{alpha}2 isoforms regulated different early-response genes when stimulated with GDNF or NTN. In coexpression studies, GFR{alpha}2b was found to inhibit ligand-induced neurite outgrowth by GFR{alpha}2a and GFR{alpha}2c. Stimulation of GFR{alpha}2b also inhibited the neurite outgrowth induced by GFR{alpha}1a, another member of the GFR{alpha}. Furthermore, activation of GFR{alpha}2b inhibited neurite outgrowth induced by retinoic acid and activated RhoA. Together, these data suggest a novel paradigm for the regulation of growth factor signaling and neurite outgrowth via an inhibitory splice variant of the receptor. Thus, depending on the expressions of specific GFR{alpha}2 receptor spliced isoforms, GDNF and NTN may promote or inhibit neurite outgrowth through the multicomponent receptor complex.

Key words: GDNF; NTN; GFR{alpha}2; RhoA; inhibitory splice isoforms; neuroblastoma


    Introduction
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Neurturin (NTN), glial cell line-derived neurotrophic factor (GDNF), Artemin, and Persephin are cysteine knot proteins and are members of the GDNF family ligands (GFLs) (Kotzbauer et al., 1996Go; Airaksinen and Saarma, 2002Go). These GFLs have been shown to support the growth, maintenance, and differentiation of a wide variety of neuronal and extraneuronal systems (Saarma and Sariola, 1999Go). Each GFL is known to bind preferentially to one GDNF family receptor {alpha} (GFR{alpha}) in vitro, and the activation of the multicomponent receptor system shows some degree of promiscuity in their ligand specificities (Horger et al., 1998Go; Airaksinen et al., 1999Go; Cik et al., 2000Go; Wang et al., 2000Go; Scott and Ibanez, 2001Go). NTN is thought to signal through its preferred receptor complex consisting of GFR{alpha}2, RET, and/or neural cell adhesion molecule (NCAM) (Baloh et al., 1997Go; Buj-Bello et al., 1997Go; Widenfalk et al., 1997Go; Paratcha et al., 2003Go).

Alternative splicing is prevalent in many mammalian genomes, as a means of producing functionally diverse polypeptides from a single gene (Blencowe, 2006Go). It has been estimated that >50% of human multi-exon genes are alternatively spliced (Modrek and Lee, 2002Go). Multiple alternatively spliced variants of GFR{alpha}1 (Sanicola et al., 1997Go; Dey et al., 1998Go; Shefelbine et al., 1998Go), GFR{alpha}2 (Wong and Too, 1998Go; Dolatshad et al., 2002Go), and GFR{alpha}4 (Lindahl et al., 2000Go, 2001Go; Masure et al., 2000Go) have been reported. Similarly, alternative spliced isoforms of the coreceptors RET (Lorenzo et al., 1997Go; de Graaff et al., 2001Go; Lee et al., 2002Go) and NCAM (Povlsen et al., 2003Go; Buttner et al., 2004Go) have been reported. The alternatively spliced isoforms of GFR{alpha}1 have recently been shown to exhibit distinct biochemical functions (Charlet-Berguerand et al., 2004Go; Yoong et al., 2005Go). These observations are consistent with the emerging view that the combinatorial interactions of the spliced isoforms of GFR{alpha}, RET, and NCAM may contribute to the multicomponent signaling system to produce the myriad of observed biological responses.

We have previously shown that all three isoforms of GFR{alpha}2 are expressed at significant levels in the murine whole brain and embryo (Too, 2003Go). It is, however, unknown whether these isoforms serve distinct or redundant functions. To gain a better insight into their biological relevance in the CNS, the expression levels of the isoforms in different regions of the human brain were quantified by highly specific real-time PCR assays. The biological functions of the isoforms were then examined in a neuronal differentiation model using Neuro2A cells and in BE(2)-C cells, which express the spliced isoforms endogenously.

Here, we showed that ligand activation of the isoforms differentially activated mitogen-activated protein kinase (MAPK) [extracellular signal-regulated kinase 1/2 (ERK1/2)] and AKT signaling and regulated distinct early-response genes. Furthermore, both GDNF and NTN induced neurite outgrowth through GFR{alpha}2a and GFR{alpha}2c, but not GFR{alpha}2b. Activation of GFR{alpha}2b inhibited neurite outgrowth induced by the other two GFR{alpha}2 isoforms as well as GFR{alpha}1a and retinoic acid. RhoA was also found to be activated by GDNF and NTN through GFR{alpha}2b. This study thus provides the first piece of evidence of a dominant inhibitory activity of GFR{alpha}2b on neurite outgrowth and distinct signaling mechanisms underlying the activation of spliced isoforms.


    Materials and Methods
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Cell culture. Neuro2A (catalog #CCL-131; American Type Culture Collection, Manassas, VA) and BE(2)-C (catalog #CRL-2268; American Type Culture Collection) cells were grown in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS; Hyclone, Logan, UT). All cultures were maintained in a 5% CO2 humidified atmosphere at 37°C.

Reverse transcription reaction. Total RNA for different human brain regions was purchased from Clontech (Palo Alto, CA). Total RNA from Neuro2A cells was prepared as described previously (Too and Maggio, 1995Go). The integrity of isolated total RNA was validated by denaturing agarose gel electrophoresis. Five micrograms of total RNA were reverse transcribed using 400 U of ImpromII and 0.5 µg of random hexamer (Promega, Madison, WI) for 60 min at 42°C according to the manufacturer's instructions. The reaction was terminated by heating at 70°C for 5 min, and the cDNA was used directly for quantitative real-time PCR. Three independent preparations of cDNA were used for the study. All measurements were performed in triplicate.

Plasmids constructions. To prepare plasmid standards for quantitative real-time PCR, open reading frames of human GFR{alpha}2 isoforms and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were subcloned into p-GEMT (Promega). For early-response genes and transcriptional factors, partial sequences were subcloned using the same primers used for real-time PCR quantification. XbaI or XmnI (Promega) was used to linearize plasmids to be used as templates for real-time PCR amplifications.

Sequence-independent real-time PCR. Real-time PCR was performed on the iCycler iQ (Bio-Rad, Hercules, CA) using SYBR Green I. The threshold cycles (Ct) were calculated using the Optical interface version 3.0B. Real-time PCR was performed after an initial denaturation for 3 min at 95°C, followed by 40–60 cycles of 60 s denaturation at 95°C, 30 s annealing at 60°C, and 60 s extension at 72°C. Fluorescent detection was performed at the annealing phase. The reaction was performed in a total volume of 50 µl in 1x XtensaMix-SG (BioWORKS, Singapore), containing 2.5 mM MgCl2, 10 pmol of primer, and 1.25 U of Platinum DNA polymerase (Invitrogen, Carlsbad, CA). Melt-curve analyses were performed at the end of PCR to verify the identity of the products. A specific exon-overlapping forward primer used for amplification of human GFR{alpha}2a was designated as "2a 15 + 9F" (5'-TCTTCTTCTTTCTAGACGAGACCC-3'), for human GFR{alpha}2b as "2b 17 + 7F" (5'-CCTCTTCTTCTTTCTAGGTGAGGA-3'), and for human GFR{alpha}2c as "2c 18 + 5F" (5'-GCCTCTTCTTCTTTCTAGGGACA-3'). A common reverse primer, designated as "553R" (5'-GCAGATGGAGATGTAGGAGGAG-3'), was used for all three isoforms. The primer pair 5'-GATCATCAGCAATGCCTCCT-3' and 5'-GCCATCACGCCACAGTTT-3' was used to amplify human GAPDH. All real-time PCR quantification was performed simultaneously with linearized plasmid standards and a nontemplate control. The gene expression levels were interpolated from standard curves and normalized to the expressions of GAPDH in the same samples. Differences in the expression levels of GFR{alpha}2 isoforms were analyzed using the paired Student's t test with a level of significance of p < 0.05.

Generation of Neuro2A cells expressing GFR{alpha}2 isoforms. The murine neuroblastoma cell line Neuro2A, which express endogenous RET and NCAM, was stably transfected with murine GFR{alpha}2a, GFR{alpha}2b, GFR{alpha}2c, or vector control pIRESneo (Clontech) using Fugene-6 (Roche, Mannheim, Germany) and selected with 0.8 mg/ml G418 (Promega), over a period of 2 months. Primers used for measuring GFR{alpha}2 isoforms, RET, and NCAM expression were as described previously (Too, 2003Go; Yoong et al., 2005Go). For coexpression studies, GFR{alpha}2a, GFR{alpha}2c, or GFR{alpha}1a was cloned into the proximal 5' multiple cloning site, whereas GFR{alpha}2b was cloned into a distal 3' multiple cloning site of the bicistronic pIRES vector (Clontech). All studies were performed with three independent clones.

Assessment of neurite outgrowth in GFR{alpha}2-transfected Neuro2A cells. Twenty thousand to 50,000 cells per well were seeded on six-well plates overnight, in DMEM supplemented with 10% FBS. Cells were then incubated with medium containing 0.5% FBS, with or without 50 ng/ml recombinant human GDNF (Biosource, Camarillo, CA) or NTN (ProSpec-Tany TechnoGene, Rehovot, Israel). Cells were incubated for 3 more days. All-trans retinoic acid (5 µM; Sigma, St. Louis, MO) was used as a positive control for inducing neurite outgrowth. Cells bearing at least one neurite twice the length of the cell bodies were scored. More than 600 cells from three different fields were counted per well. Statistical significance between ligand-stimulated and control samples was calculated using the paired Student's t test. A value of p < 0.05 was considered significant.

Immunocytochemistry and confocal microscopy. Cells were seeded on chamber slides, fixed with 4% paraformaldehyde in 1x PBS for 15 min at 37°C, and subsequently fixed in methanol at –20°C for an additional 15 min. After three washes with 1x PBS, cells were permeabilized and blocked with serum (1:10; Dako, Glostrup, Denmark) and 0.5% Triton X-100 in 1x PBS for 30 min at room temperature. The cells were then incubated with F-actin (phalloidin-conjugated tetramethylrhodamine isothiocyanate) and high-molecular-weight neurofilament protein (NF-200) antibody (Sigma) in 0.1% Triton X-100, 0.1% BSA, and 1x PBS for 1 h at 37°C and washed three times in 1x PBS. A secondary antibody (Alexa Fluor 488; Invitrogen, Eugene, OR) was then added at a dilution of 1:200 and incubated for 1 h. The cells were then washed in 1x PBS and mounted for visualization. Image acquisition was obtained using a Zeiss (Oberkochen, Germany) 510 meta confocal microscope equipped with fluorescence detection.

Immunoblotting. Phosphorylation of MAPK (ERK1/2) or Akt was analyzed as follows. Cells were initially seeded in DMEM with 10% FBS for 24 h, and serum was depleted (0.5% FBS) for 16 h. The cells were then treated with 50 ng/ml GDNF, NTN, Artemin, or Persephin (PreproTech, London, UK) in serum-free medium for different periods of time at 37°C. For dose–response studies, cells were stimulated with different concentrations of ligands for 10 min at 37°C. Control treatment with 1 M Sorbitol (Sigma) was performed simultaneously. The supernatants were then removed, and cells were washed once with 1x PBS and subsequently lysed in 2% SDS. Protein concentrations were estimated using the BCA assay (Pierce, Rockford, IL). ERK1/2 or Akt phosphorylation was analyzed by Western blot using phospho-specific antibodies according to the manufacturer's instructions (Cell Signaling Technology, Danvers, MA). Blots were stripped with Restore Western Stripping Buffer (Pierce) and reprobed with pan antibodies to verify equal loading of protein.

For studying kinetics and dose–response of ligand-induced ERK1/2 activation, dot blot analysis was performed using the BIO Dot Apparatus (Bio-Rad). Five micrograms of protein were loaded per well in triplicates. Blots were then detected by anti-phospho ERK1/2 antibodies (Cell Signaling Technology) according to the manufacturer's instructions. Densities of blots were imaged and measured by Quantity One 4.0 (Bio-Rad).

Binding of [125I]GDNF to GFR{alpha}2 isoforms transfected Neuro2A cells. [125I] GDNF (~1000 mCi/mmol) was prepared using Bolton and Hunter reagent (Amersham Biosciences, Piscataway, NJ). Briefly, 10 µg of recombinant human GDNF (Biosource) was labeled with 1 mCi of Bolton and Hunter reagent for 1 h at room temperature according to the manufacturer's instructions. The reaction was then terminated by adding 10 µl of 0.1% tyrosine. Radiolabeled GDNF was then purified on a Sephadex G-10 column.

Binding studies were performed as described previously (Jing et al., 1997Go). Briefly, 0.1 million cells were seeded per well on 24-well Costar (Cambridge, MA) tissue culture plates for 2 d before the assay. Before the experiment, cells were placed on ice for 15–20 min and washed once with ice-cold DMEM buffer and 25 mM HEPES, pH 7.0. Cells were then incubated at 4°C for 3 h with 0.2 ml of binding buffer [DMEM, 25 mM HEPES, 2 mg/ml bovine albumin serum, and Complete Inhibitor Cocktail (Roche), pH 7.0] containing 50 pM [125I]GDNF and various concentrations of unlabeled GDNF. At the end of incubation, cells were washed three times with 0.3 ml of ice-cold washing buffer and lysed in 0.1% SDS containing 1 M NaOH. The radioactivity in lysates was measured using the auto gamma counter (PerkinElmer Packard, Wellesley, MA).

Measurements of early-response genes regulated by GDNF and NTN. Cells were seeded in DMEM with 10% FBS for 24 h, followed by serum depletion (0.5% FBS) for 18–24 h. The cells were then treated with GDNF (50 ng/ml) or NTN (50 ng/ml) in serum-free medium for varying periods of time at 37°C. Total RNA was then isolated and reverse transcribed as described above. The gene expression levels were then quantified by real-time PCR using gene-specific primers. Primers used for amplification of early response genes were as follows: EGR-1-328F/EGR-1-459R (5'-GAGAAGGCGATGGTGGAGACGA-3'/5'-GCTGAAAAGGGGTTCAGGCCA-3') for egr-I; EGR-2-1F/EGR-2-179R (5'-ATGAACGGAGTGGCGGGAGAT-3'/5'-TCTGGATAGCAGCTGGCACCAG-3') for egr-2; mcfos(B)651F/mcfos(B)901R (5'-TGTGGCCTCCCTGGATTT-3'/5'-CTGCATAGAAGGAACCGGAC-3') for c-fos; and mFosB(A)1926F/mFosB(A)2107R (5'-CAGGGTCAACATCCGCTAA-3'/5'-GGAAGTGTACGAAGGGCTAACA-3') for fosB. Expression of target genes and GAPDH was interpolated from standard curves. The fold change of each target gene was calculated as a change in gene expression of the stimulated sample normalized to GAPDH compared with gene expression of the control sample normalized to GAPDH.

Silencing of GFR{alpha}2b in BE(2)-C. Small interfering RNA (siRNA) duplexes (Invitrogen) were designed across specific exon (exons 1 and 3) boundaries of GFR{alpha}2b (siGFR{alpha}2b-15+5: TCTTCTTCTTTCTAGGTGAG; siGFR{alpha}2b-13+7: TCTTCTTCTTTCTAGGTGAGGA; siGFR{alpha}2b-10+10: TTCTTTCTAGGTGAGGAGTT; siGFR{alpha}2b-7+13: TTTCTAGGTGAGGAGTTCTA; siGFR{alpha}2b-5+15: TCTAGGTGAGGAGTTCTACG). Subconfluent cells (50–80%) were seeded on six-well plates, in 10% FBS DMEM. Cells were transfected with siRNA duplexes (20 pmol) using Transfectin (Bio-Rad) in 400 µl of 0.5% FBS DMEM per well. Total RNA was isolated 6 h after transfection, and gene expression was measured by real-time PCR. For differentiation studies using BE(2)-C, 6 h after silencing of GFR{alpha}2b, 2 ml of differentiation medium containing retinoic acid (5 µM), GDNF (50 ng/ml), or NTN (50 ng/ml) in 0.5% FBS DMEM was added to the medium. Analyses of morphological differences were performed after 3 d.

RhoA assay. Neuro2A cells were seeded in 10% FBS DMEM and incubated for 18–24 h. Subsequently, the serum was reduced to 0.5% in DMEM, and the cells were incubated for an additional 18–24 h. Cells were then treated with 10 µM lysophosphatidic acid (LPA; Sigma), GDNF (50 ng/ml), or NTN (50 ng/ml) in serum-free DMEM for 10 min. Cells were lysed and used directly for the GTP-RhoA pull-down assay according to the manufacturer's instructions (Pierce). RhoA inhibitor exoenzyme C3 transferase and Rho kinase (ROCK) inhibitor Y27632 were purchased from Calbiochem (La Jolla, CA). Exoenzyme C3 transferase was transfected into cells using the lipotransfecting agent Transfectin (Bio-Rad), at 1 µl of Transfectin/1 µg of C3 transferase per well of a six-well plate, 4 h before start of the experiment. Cells were then treated with RhoA inhibitor exoenzyme C3 transferase (1 µg/ml) or ROCK inhibitor Y27632 (10 µM), in the presence or absence of differentiating stimuli. LPA (10 µM; Sigma) was used as a positive control for activities of Rho and ROCK inhibitor.


    Results
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Differential expression profiles of GFR{alpha}2 spliced variants
Currently, the expression levels of GFR{alpha}2 spliced variants in specific regions of the brain are unknown. To address this issue, we have developed sequence-independent real-time PCR assays to quantify each of the spliced variants with high specificity and sensitivity.

To discriminate between the three spliced variants of human GFR{alpha}2, overlapping exon primers were designed across exons 1 and 2, 1 and 3, or 1 and 4 to enable the specific detection and quantification of GFR{alpha}2a, GFR{alpha}2b, and GFR{alpha}2c, respectively (Fig. 1A). Because the amplification products of GFR{alpha}2a (545 bp), GFR{alpha}2b (233 bp), and GFR{alpha}2c (172 bp) were different in sizes, it was critical to determine the optimal cycling parameters for the selective amplification of each of the transcripts. A dwell time of 30 s for annealing, 60 s for denaturation at 95°C, and 60 s for extension at 72°C were found to be optimal for the amplifications of all three isoforms. The slopes of the plots of Ct versus log10 mole of the human GFR{alpha}2a, GFR{alpha}2b, and GFR{alpha}2c standards were 3.37 ± 0.30 (r2 = 0.98), 4.12 ± 0.41 (r2 = 0.99), and 3.82 ± 0.54 (r2 = 0.99), respectively. The samples diluted in parallel with the standards (data not shown). The specificity of amplifying a particular isoform compared with the other variants was >106-fold (data not shown). Hence, the amplifications of GFR{alpha}2b and GFR{alpha}2c were at least 106-fold less efficient than amplifying GFR{alpha}2a, when using GFR{alpha}2a exon-overlapping primers. The detection limits of the assays were estimated to be <100 copies of transcripts per reaction.


Figure 1
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Figure 1. Real-time PCR quantification of the expressions of GFR{alpha}2 isoforms in the human brain. A, A schematic diagram showing the protein coding exons and the positions of the primers used for quantitative real-time PCR. Exons 1–9 encode the full-length protein sequence of GFR{alpha}2a. Specific forward primers (2a 15 + 9F for GFR{alpha}2a, 2b 17 + 7F for GFR{alpha}2b, and 2c 18 + 5F for GFR{alpha}2c) were designed across exon junctions, whereas a common reverse primer (553R) was used for the amplification of all the three isoforms. B, The expression levels of GFR{alpha}2 isoforms in a different human brain region normalized to the levels of GAPDH in the same tissue. The results were expressed as mean ± SEM (n = 3). Significant differences between the expressions of the isoforms were calculated using paired Student's t test. A value of p < 0.05 was considered significant (**p = 0.001).

 
Using these highly sensitive and specific assays, the expression levels of the GFR{alpha}2 alternatively spliced isoforms were quantified in caudate nucleus, cortex, putamen, substantia nigra, subthalamic nucleus, and thalamus of the human brain (Fig. 1B). The three GFR{alpha}2 isoforms were detected at significant levels (>104 copies per reaction) in all areas of the brain, with expression levels highest in the cortex. In cortex, all three isoforms were expressed at comparable levels, with GFR{alpha}2b expression significantly lower than GFR{alpha}2c (p < 0.01).

GFR{alpha}2 isoforms differentially activated ERK1/2 and Akt
To investigate the biological significance of alternatively spliced GFR{alpha}2 isoforms, stable transfectants were generated in Neuro2a cells. We have shown previously that Neuro2a cells express RET and NCAM, but not GFR{alpha}2 receptors, endogenously (Yoong et al., 2005Go). The expression levels of GFR{alpha}2 isoforms in stably transfected Neuro2a cells (supplemental Fig. 1, available at www.jneurosci.org as supplemental material) were comparable to that expressed in the human cortex (Fig. 1B).

When stimulated with NTN, all the three isoforms induced the rapid phosphorylation of ERK1/2 (Fig. 2A). However, when stimulated with GDNF, GFR{alpha}2a (Fig. 2A,C) and GFR{alpha}2c (Fig. 2A,E), but not GFR{alpha}2b (Fig. 2A,D), induced significant ERK1/2 phosphorylation (more than twofold). The extent of ERK1/2 phosphorylation was similar when GFR{alpha}2a (Fig. 2C) and GFR{alpha}2c (Fig. 2E) activated with either GDNF or NTN. However, GFR{alpha}2b showed rapid and significant phosphorylation of ERK1/2 only with NTN stimulation but not by GDNF (Fig. 2A,D). Both GDNF and NTN induced ERK1/2 phosphorylation in a dose–response manner in GFR{alpha}2a (Fig. 2F) and GFR{alpha}2c (Fig. 2H) transfectants. GDNF appeared to be slightly more potent than NTN in inducing ERK1/2 phosphorylation in both transfectants (Fig. 2F,H). Compared with the stimulation with NTN, GFR{alpha}2b when stimulated with GDNF showed no significant increase in ERK1/2 phosphorylation even at the highest dose (Fig. 2G). No significant increase in the phosphorylation of ERK1/2 was observed in vector (pIRESneo) control transfected Neuro2A cells when stimulated with either GDNF or NTN (data not shown).


Figure 2
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Figure 2. Activations of ERK1/2 and Akt in GFR{alpha}2 isoforms transfected Neuro2A cells when stimulated by either GDNF or NTN. Cells were stimulated in serum-free medium, with or without GDNF or NTN (50 ng/ml), for the period of time indicated. A, B, Five micrograms of protein were loaded and separated by SDS electrophoresis, and phosphorylated ERK1/2 (pERK1/2; A) or Akt (pAkt; B) was then detected by Western blot. Blots were stripped and reprobed with pan antibody as loading controls. C–E, Kinetics of GDNF and NTN induced ERK1/2 activations in GFR{alpha}2a (C), GFR{alpha}2b (D), and GFR{alpha}2c (E). Cells were treated with 50 ng/ml GDNF or NTN for 5, 15, and 30 min. F–H, Dose responses of the activation of ERK1/2 when stimulated with GDNF or NTN in GFR{alpha}2a (F), GFR{alpha}2b (G), and GFR{alpha}2c (H) isoforms. Cells were stimulated for 10 min with ligand at various doses. For kinetic and dose–response studies, 5 µg of protein was loaded per well for dot blot quantification of phospho-ERK1/2 (pERK1/2). The means ± SD were calculated from results obtained in triplicates. Significant differences in fold change of pERK1/2 between ligand stimulated and control were calculated using the paired Student's t test. A value of p < 0.05 was considered significant (**p < 0.001; *p < 0.05). Experiments were repeated three times with two independent clones with similar results.

 
We next investigated the ligand-regulated phosphorylation of Akt using GDNF or NTN (Fig. 2B). NTN induced rapid and significant phosphorylations of Akt in all three isoform transfectants. However, GDNF induced the rapid and significant phosphorylations of Akt in cells expressing GFR{alpha}2b and GFR{alpha}2c.

The other GFLs, Artemin and Persephin, did not induce significant phosphorylation of ERK1/2 or Akt in any of the GFR{alpha}2 isoform transfectants (data not shown). In addition, neither GDNF nor NTN was found to activate p38 and c-Jun N-terminal kinase (JNK) in any of the GFR{alpha}2 isoform transfectants, even at concentrations as high as 100 ng/ml and over a period of 1 h of ligand stimulation (data not shown).

[125I]GDNF bound equally well to all three GFR{alpha}2 isoforms
NTN has been shown to bind with similar affinities to the GFR{alpha}2 isoforms (Scott and Ibanez, 2001Go). Because GDNF failed to induce a significant increase in the phosphorylation of ERK1/2 in GFR{alpha}2b transfectants (Fig. 2A,D,G), it is possible that GDNF may not bind to this isoform. To address this possibility, we next performed a ligand displacement study using [125I]GDNF (supplemental Fig. 2, available at www.jneurosci.org as supplemental material). GDNF displaced the binding of [125I]GDNF to the three GFR{alpha}2 isoforms with similar potencies. The IC50 for the displacements of cells transfected with GFR{alpha}2a, GFR{alpha}2b, and GFR{alpha}2c were 3.27 ± 0.02, 2.79 ± 0.16, and 2.31 ± 0.09 nM (mean ± SD), respectively. Parental Neuro2A or cells transfected with pIRESneo showed no significant binding to [125I]GDNF. This result indicates that GDNF binds to all three isoforms with similar affinities.

GFR{alpha}2 isoforms activated different transcriptional genes
The differential activation of ERK1/2 and Akt (Fig. 2) suggests the possibility that downstream biochemical mechanisms may differ. To explore this issue, we measured the changes in gene expression of the fos family (c-fos, fosB), jun family (c-jun, jun-b), egr family (egr1–4), and GDNF-inducible transcription factors mGIF and mGZF1 in response to GDNF and NTN (supplemental Table 1, available at www.jneurosci.org as supplemental material). These factors have previously been shown to be activated with GDNF or NTN (Yajima et al., 1997Go; Trupp et al., 1999Go; Kozlowski et al., 2000Go; Fukuda et al., 2003Go; Pezeshki et al., 2003Go). The kinetics of gene activations over a period of 6 h was quantified by real-time PCR (Fig. 3). Distinct ligand-induced early-response gene expressions were observed with the activation of the different GFR{alpha}2 isoforms. GFR{alpha}2a, when stimulated by GDNF (Fig. 3A) or NTN (Fig. 3B), upregulated egr-1 by as much as fourfold to fivefold. GFR{alpha}2b, when stimulated by GDNF (Fig. 3C) or NTN (Fig. 3D), upregulated fosB by >10-fold compared with control. When stimulated with GDNF (Fig. 3E) or NTN (Fig. 3F), GFR{alpha}2c upregulated the expressions of egr-1 and egr-2. With the other genes, no significant changes were observed with GDNF or NTN stimulations. These results showed that the activation of GFR{alpha}2b isoform regulates the transcription of specific sets of early-response genes.


Figure 3
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Figure 3. Kinetic analyses of the regulations of early-response genes by GDNF and NTN in GFR{alpha}2 isoform transfectants. The fold change of mRNA expressions of early-response genes in cells expressing GFR{alpha}2a (A), GFR{alpha}2b (C), and GFR{alpha}2c (E) when stimulated with GDNF and GFR{alpha}2a (B), GFR{alpha}2b (D), and GFR{alpha}2c (F) when stimulated with NTN at the designated period of time is shown. The expression levels were measured by quantitative real-time PCR. Similar results were obtained from more than three separate experiments. Error bars indicate SDs of triplicate measurements from one study. Significant differences in expression of genes between ligand stimulated and control were calculated using the paired Student's t test. A value of p < 0.05 was considered significant (**p < 0.001; *p < 0.05).

 
Neurite outgrowths were induced by GFR{alpha}2a and GFR{alpha}2c, but not GFR{alpha}2b
Neuro2a cells serve as an excellent in vitro model system for studying signaling pathways mediating neurite outgrowth. Under normal growth conditions, most Neuro2a cells spontaneously sprout a basal level of neurites. However, treatment with a variety of stimuli cause these cells to develop extensive neurites similar to changes observed in hippocampal and cortical cultures (Ahmari et al., 2000Go; Washbourne et al., 2002Go).

To investigate possible morphological changes induced by the activation of the GFR{alpha}2 isoforms, the transfectants were stimulated with either GDNF or NTN. Both GFR{alpha}2a and GFR{alpha}2c transfectants showed extensive neurite outgrowths when stimulated with either ligand, comparable to the effects of retinoic acid (Fig. 4). Unexpectedly, neither NTN nor GDNF induced neurite outgrowth in cells expressing GFR{alpha}2b (Fig. 4). Immunocytochemical staining for ß-III tubulin confirmed these observations. (supplemental Fig. 3, available at www.jneurosci.org as supplemental material). Cells expressing GFR{alpha}2b extended neurite-like structures when treated with retinoic acid, indicative of the potential for neurite outgrowth (Fig. 4A). GDNF and NTN have no neuritogenic effect on control vector-transfected Neuro2A cells (Fig. 4).


Figure 4
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Figure 4. Differential neuritogenic activities of ligand-activated GFR{alpha}2 isoforms. Cells were seeded on six-well plates and incubated for 16–18 h in medium containing 10% serum. The cells were then exposed to GDNF or NTN (50 ng/ml) for 3 more days in 0.5% serum-containing medium. Retinoic acid (5 µM) was used as a positive control for cell differentiation. A, Digital phase-contrast images of Neuro2A cells stably expressing GFR{alpha}2a, GFR{alpha}2b, GFR{alpha}2c, or pIRES vector control when treated with retinoic acid, GDNF, or NTN. B, Percentages of cells bearing neurites that were at least twice the length of the cells bodies. More than 600 cells were counted per well, on at least three different fields. Experiments were repeated twice with three individual clones, with similar results. Significant differences in the percentage of cells bearing neurites between ligand stimulated and control were calculated using the paired Student's t test (*p < 0.002). Error bars indicate mean ± SD of triplicate measurements. RA, Retinoic acid.

 
To further examine the morphological changes in these cells, two major cytoskeletal components, F-actin and high-molecular-weight neurofilament protein (NF-H), which are involved in neurite outgrowth dynamics, were visualized by fluorescent staining (Myers et al., 2006Go). With ligand (GDNF or NTN)-stimulated GFR{alpha}2a and GFR{alpha}2c transfectants, NF-H-positive filopodia (axon-like processes) were relatively long and formed thick threads. Protrusions with F-actin staining were observed at the edges of the thick NF-H-positive axon-like elements and cell bodies (supplemental Fig. 4C, arrowheads, available at www.jneurosci.org as supplemental material). Engorgements were seen at some terminal structures that were both NF-H and F-actin positive (supplemental Fig. 4C, arrow, available at www.jneurosci.org as supplemental material). Long extensions were not obvious with cells expressing GFR{alpha}2b when stimulated with either ligand. Instead, F-actin-positive staining was found at the periphery of these cells where NF-H was not found to colocalize extensively (supplemental Fig. 4F, available at www.jneurosci.org as supplemental material). These observations provide additional evidence of the lack of neurite outgrowth in ligand-stimulated cells expressing GFR{alpha}2b and the neuritogenic activities of the other two isoforms.

GFR{alpha}2b inhibited neurite outgrowth mediated by GFR{alpha}2a, GFR{alpha}2c, and GFR{alpha}1a isoform
Because GFR{alpha}2b transfectants did not induce neurite outgrowth when stimulated with ligands, we explored the possibility that this isoform may affect the morphological changes in cells coexpressing GFR{alpha}2b and GFR{alpha}2a or GFR{alpha}2c. We first established stably transfected Neuro2A cells coexpressing GFR{alpha}2a and GFR{alpha}2b (GFR{alpha}2a + GFR{alpha}2b) using a bicistronic vector. Expression and membrane targeting of GFR{alpha}2a were not affected when coexpressed with GFR{alpha}2b (supplemental Fig. 5, available at www.jneurosci.org as supplemental material). As shown previously, ligand-induced stimulation of GFR{alpha}2a but not GFR{alpha}2b induced neurite outgrowth (Fig. 4B). Ligand-induced stimulation of cells coexpressing GFR{alpha}2a + GFR{alpha}2b showed significantly less neurite outgrowth (Fig. 5A). However, these cells extended neurite when treated with retinoic acid. Similarly, ligand stimulation of cells coexpressing GFR{alpha}2c and GFR{alpha}2b (GFR{alpha}2c + GFR{alpha}2b) showed significantly less neurite outgrowth (Fig. 5A).


Figure 5
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Figure 5. Ligand-induced neurite outgrowth in Neuro2A coexpressing GFR{alpha}2b with other GFR{alpha} isoforms. GFR{alpha}2b was stably coexpressed with GFR{alpha}2a (GFR{alpha}2a + GFR{alpha}2b) or GFR{alpha}2c (GFR{alpha}2c + GFR{alpha}2b) (A) or GFR{alpha}1a (GFR{alpha}1a + GFR{alpha}2b) (B). Cells were treated with or without GDNF or NTN (50 ng/ml) for 3 d in 0.5% serum-containing medium. Retinoic acid (RA; 5 µM) differentiated all the transfectants efficiently. Experiments have been repeated twice with three independent clones, with similar results. Significant differences in the percentage of cells bearing neurites between ligand stimulated and control were calculated using the paired Student's t test (**p < 0.002; *p = 0.05). Error bars indicate mean ± SD of triplicate measurements.

 
Extending this finding, we next explored the possible inhibitory effect of the activation of GFR{alpha}2b on the neurite outgrowth induced by ligands in cells coexpressing GFR{alpha}1a. Cells expressing only GFR{alpha}1a showed significant neurite outgrowth when stimulated by GDNF, NTN, or retinoic acid (Fig. 5B). Interestingly, when stimulated by either GDNF or NTN, cells coexpressing GFR{alpha}1a and GFR{alpha}2b (GFR{alpha}1a + GFR{alpha}2b) showed significantly less neurite outgrowth. These observations indicate that the activation of GFR{alpha}2b inhibits neurite outgrowth induced by the activation of GFR{alpha}2a, GFR{alpha}2c, and even the structurally related GFR{alpha}1a.

Knock-down of GFR{alpha}2b resulted in an increase in neurite outgrowth
We next extended the above observation of the GFR{alpha}2b-induced inhibition of neurite outgrowth by investigating BE(2)-C cells that have previously been shown to endogenously express GFR{alpha}2 receptors (Kobori et al., 2004Go). These cells express a comparable level of GFR{alpha}2a and GFR{alpha}2b (Fig. 6A). GFR{alpha}2c was found to be expressed at a level close to the detection limit of the assay (data not shown). The presence of GFR{alpha}2 but not GFR{alpha}1 in BE(2)-C cells agrees with previous observations (Kobori et al., 2004Go; Yoong et al., 2006Go) but not with a recent report using semiquantitative PCR (Hansford and Marshall, 2005Go).


Figure 6
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Figure 6. Silencing of GFR{alpha}2b expression in human BE(2)-C cells. A, The expression levels of GFR{alpha}2a and GFR{alpha}2b in BE(2)-C cells were determined using quantitative real-time PCR. B, Effects of various designs of siRNA sequences on the expressions of GFR{alpha}2a and GFR{alpha}2b in BE(2)-C. siRNA duplex (20 pmol) was transfected into cells, and total RNA was harvested 6 h later. The expressions of GFR{alpha}2a and GFR{alpha}2b were then measured by quantitative real-time PCR. Significant differences between the expression of the two isoforms after silencing with each of the siRNA designs were calculated using the paired Student's t test (**p = 0.001). C, D, Neurite outgrowth of BE(2)-C cells after silencing of GFR{alpha}2b. C, Top row, Cells were stimulated with retinoic acid (5 µM), GDNF, or NTN (50 ng/ml) in the absence of siRNA. Bottom row, Cells were transfected with siGFR{alpha}2b-13+7 for 6 h and subsequently stimulated with retinoic acid (5 µM), GDNF, or NTN (50 ng/ml). Pretreament of cells with siGFR{alpha}2b-13+7 and subsequent stimulation with GDNF or NTN resulted in the formation of neurite-like structures (arrows). D, Percentages of cells bearing neurites that were at least twice the length of the cells bodies were scored in the presence (+) or absence (–) of the siRNA siGFR{alpha}2b-13+7. Similar results were obtained from replicates of three individual experiments. Significant differences in the percentage of cells bearing neurites between ligand stimulated and control were calculated using the paired Student's t test (**p < 0.002; *p = 0.05). Error bars indicate mean ± SD of triplicate measurements. RA, Retinoic acid.

 
Similar to the above observations with the coexpression of GFR{alpha}2b with GFR{alpha}2a, GFR{alpha}2c, or GFR{alpha}1a, both GDNF and NTN failed to induce neurite outgrowth in BE(2)-C cells (Fig. 6C, control). Neurite outgrowth was, however, observed when these cells were treated with retinoic acid, an indication that BE(2)-C cells have the capability of forming neurite-like structures. To test the hypothesis that the activation of GFR{alpha}2b may inhibit neurite outgrowth induced by GFR{alpha}2a or GFR{alpha}2c in BE(2)-C cells, we attempted to silence the expression of GFR{alpha}2b using siRNA. Because GFR{alpha}2b has no unique sequences compared with GFR{alpha}2a, the design of a GFR{alpha}2b isoform-specific siRNA poses a significant challenge. A series of siRNA duplexes were then designed with sequences overlapping exons 1 and 3 of GFR{alpha}2b (Fig. 6B). Of the five designs tested, only the siGFR{alpha}2b-13+7 showed significant discrimination in silencing GFR{alpha}2a and GFR{alpha}2b (Fig. 6B). This particular siRNA design, siGFR{alpha}2b-13+7, inhibited the expression of GFR{alpha}2b to <10% of the control, with no significant reduction in the expression of GFR{alpha}2a.

When GFR{alpha}2b expression was silenced, the BE(2)-C cells extended neurite-like structures when stimulated with either GDNF and NTN (Fig. 6C). This observation supports the notion that the activation of GFR{alpha}2b inhibits neurite outgrowth induced by ligand stimulation of GFR{alpha}2a.

Signaling and biochemical activities of GFR{alpha}2 isoforms in the co-expression model
To further investigate the signaling and biochemical events underlying ligand activation of GFR{alpha}2b in the coexpression model, we first examined the stimulation of MAPK (ERK1/2). GDNF stimulated ERK1/2 phosphorylation in GFR{alpha}2a or GFR{alpha}2c, but not GFR{alpha}2b, transfectants (Fig. 2A). In the coexpression models, both GDNF and NTN induced rapid and transient phosphorylation of ERK1/2 (Fig. 7A).


Figure 7
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Figure 7. Ligand-regulated ERK1/2 signaling and expressions of immediate-early-response genes in Neuro2A coexpressing GFR{alpha}2b and the other GFR{alpha}2 isoforms. A, Western blot analyses of the activation of ERK1/2. Neuro2A cells stably coexpressing the isoforms GFR{alpha}2a and GFR{alpha}2b (GFR{alpha}2a + GFR{alpha}2b) or GFR{alpha}2c and GFR{alpha}2b (GFR{alpha}2c + GFR{alpha}2b) were treated with GDNF, NTN, or Sorbitol for the period of time indicated. Phospho-specific antibodies to ERK1/2 were used for detection, and the blots were reprobed with pan antibody serving as controls for protein loadings. B–E, Kinetic analyses of GDNF- or NTN-regulated expressions of early-response genes in the coexpression models. Expressions of fosB, egr-1, and egr-2 were measured with quantitative real-time PCR in cells stably coexpressing GFR{alpha}2a with GFR{alpha}2b (GFR{alpha}2a + GFR{alpha}2b) when stimulated with GDNF (B) or NTN (C); cells stably coexpressing GFR{alpha}2c with GFR{alpha}2b (GFR{alpha}2c + GFR{alpha}2b) were stimulated with GDNF (D) or NTN (E). Significant differences in expression of genes between ligand stimulated and control were calculated using the paired Student's t test. A value of p < 0.05 was considered significant (**p < 0.001).

 
Interestingly, when stimulated with either GDNF or NTN, no change in the expression of either egr-1 or egr-2 was observed (Fig. 7B–E). However, significant upregulation of the expression of fosB was observed in the coexpression of GFR{alpha}2b with GFR{alpha}2a (GFR{alpha}2a + GFR{alpha}2b) or with GFR{alpha}2c (GFR{alpha}2b + GFR{alpha}2c). This observation showed that the activation of coexpressed GFR{alpha}2b with either GFR{alpha}2a or GFR{alpha}2c results in the activation of fosB, an early-response gene, reminiscent of that observed in GFR{alpha}2b transfected alone (Fig. 3).

GFR{alpha}2b also inhibited retinoic acid-induced neurite outgrowth and activated RhoA
We next addressed the possibility that GFR{alpha}2b may affect neurite outgrowth induced by retinoic acid, a non-GFL stimulus. Using Neuro2A-expressing GFR{alpha}2b, retinoic acid treatment resulted in extensive neurite outgrowth. Both GDNF and NTN dramatically reduced the number of cells bearing neurite-like structures in retinoic acid-treated GFR{alpha}2b transfectant (Fig. 8).


Figure 8
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Figure 8. Ligand-activated GFR{alpha}2b antagonizes the neurite outgrowth induced by retinoic acid (RA). RA (5 µM) induced neurite outgrowth in GFR{alpha}2b-expressing Neuro2A cells. When treated together with GDNF or NTN (50 ng/ml), neurite outgrowth induced by RA was significantly attenuated. A, Phase-contrast images of Neuro2A cells stably expressing GFR{alpha}2b, treated with RA, GDNF, or NTN for 3 d. B, Graph of the percentage of cells bearing neurite with at least two times the length of the cell bodies and the effects of RA, GDNF, and NTN. Similar results were obtained from three independent clones. Significant differences in the percentage of cells bearing neurites between ligand stimulated and control were calculated using the paired Student's t test (*p < 0.002). Error bars indicate mean ± SD of triplicate measurements.

 
The Rho family of small GTPases and the associated regulators have been implicated in the modulation of neurite formation, axonal pathfinding, and dendritic arborization (Mackay et al., 1997Go; Van Aelst and Cline, 2004Go). Thus, it was of interest to examine the possibility that GFR{alpha}2b may activate the Rho family of GTPases. When stimulated with either GDNF or NTN, Neuro2a coexpressing GFR{alpha}2a and GFR{alpha}2b (GFR{alpha}2a + GFR{alpha}2b) or GFR{alpha}2c and GFR{alpha}2b (GFR{alpha}2c + GFR{alpha}2b) did not extend neurite-like structures (Fig. 5A). However, a significant number of these cells extended neurite-like structures in the presence of C3 transferase, suggesting the involvement of the Rho family of GTPases in the inhibitory effects of GFR{alpha}2b (Fig. 9A,B). Because Neuro2A cells have previously been shown to respond to LPA, resulting in the inhibition of neurite outgrowths through the RhoA-dependent mechanism (Sayas et al., 2002Go), it was not surprising that C3 transferase was found to inhibit LPA effects on retinoic acid-induced neurite outgrowth. At the concentration of C3 transferase used in this study, no significant cell death was observed (data not shown).


Figure 9
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Figure 9. Effects of RhoA and ROCK inhibitors in ligand-induced neurite outgrowth of GFR{alpha}2 isoform coexpression models and the ligand-induced activation of RhoA in GFR{alpha}2 isoform transfectants. A, B, Effects of RhoA inhibitor exoenzyme C3 transferase (1 µg/ml) and ROCK inhibitor Y27632 (10 µM) on ligand-induced neurite outgrowth in coexpression models of GFR{alpha}2a and GFR{alpha}2b (A) or GFR{alpha}2c and GFR{alpha}2b (B). LPA was used as a positive control in this study. LPA (10 µM) antagonizes neurite outgrowth induced by 5 µM retinoic acid (RA); such neurite inhibition of LPA was attenuated by C3 (1 µg/ml) and Y27632 (10 µM). The means ± SD were calculated from results obtained in triplicates. The effects of RhoA and ROCK inhibitors were compared with the effects of the inhibitors alone. With the concentrations of inhibitors used, no significant cell deaths were observed. Significant differences in the percentage of cells bearing neurites were calculated between ligand stimulated and control, using the paired Student's t test (*p ≤ 0.01). C, Analyses of RhoA activation in Neuro2A cells transfected with GFR{alpha}2 isoforms or pIRES control. After a 10 min pretreatment of LPA (10 µM), GDNF, or NTN (50 ng/ml), GTP-bound RhoA was pulled down from cell lysates using GST–Rhotekin and immunoblotted for RhoA. LPA served as a positive control for RhoA activation. Blotting of total RhoA in cell lysates showed similar loading of cell lysates.

 
To gain a better understanding of the mechanisms underlying the inhibitory effects of GFR{alpha}2b, we next examined the possible involvement of ROCK, which is known to be an effector of RhoA in the negative regulation of neurite outgrowth (Dickson, 2001Go; Sayas et al., 2002Go). Using the ROCK inhibitor Y27632, the inhibitory activity of LPA on retinoic acid-induced neurite outgrowth was significantly attenuated (Fig. 9A,B). However, the same concentration of Y27632 (10 µM) did not attenuate the inhibitory activity of GFR{alpha}2b (Fig. 9A,B). Higher concentrations of Y27632 (20 µM) resulted in significantly higher background neurite outgrowth and therefore complicated the interpretation of the study.

To investigate the possible involvement of RhoA in the inhibitory effects of GFR{alpha}2b, an attempt was made to pull down activated RhoA from cells lysates using glutathione S-transferase (GST)–Rhotekin and subsequently immunoblotted for RhoA. Similar to the effects of LPA, GFR{alpha}2b, when stimulated with either NTN or GDNF, was found to activate RhoA significantly (Fig. 9C). However, Neuro2A expressing GFR{alpha}2a, GFR{alpha}2c, or pIRES vector control did not activate RhoA significantly when stimulated with these ligands. This observation is consistent with the suggestion that RhoA and/or other Rho GTPases may be involved in the inhibition of neurite outgrowth mediated through GFR{alpha}2b.

The involvement of Rho in the activation of GFR{alpha}2b is not restricted to inhibiting GFR{alpha}1a-, GFR{alpha}2a-, or GFR{alpha}2c-induced neurite outgrowth but also to that induced by retinoic acid (Fig. 10A). Similar to the above observations, the inhibitory effects of GFR{alpha}2b on retinoic acid-induced neurite outgrowth appeared to be mediated through a Rho-dependent manner. Furthermore, the inhibition of ROCK may be sufficient to oppose the effects of LPA but not that of GFR{alpha}2b on retinoic acid-induced neurite outgrowth (Fig. 10B).


Figure 10
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Figure 10. Effects of RhoA and ROCK inhibitors on GFR{alpha}2b inhibition of retinoic acid (RA)-induced neurite outgrowth. A, RhoA inhibitor exoenzyme C3 transferase (1 µg/ml) inhibited the ligand-activated GFR{alpha}2b attenuation of neurite extension induced by RA (5 µM). The same concentration of exoenzyme C3 transferase also attenuated LPA (10 µM) inhibition of RA-induced neurite extension. B, Lack of effect of ROCK inhibitor Y27632 on the ligand-activated GFR{alpha}2b inhibition of RA induced neurite extension. The same concentration of Y27632 (10 µM) significantly attenuated the neurite outgrowth inhibition induced by LPA. The means ± SD were calculated from results obtained in triplicates. The effects of RhoA and ROCK inhibitors were compared with the effects of the inhibitors alone. With the concentrations of inhibitors used, no significant cell deaths were observed. Significant differences in the percentage of cells bearing neurites were calculated between ligand stimulated and control, using the paired Student's t test (*p ≤ 0.01).

 

    Discussion
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 Abstract
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 Materials and Methods
 Results
 Discussion
 References
 
This study demonstrates a novel function of GFR{alpha}2b, an alternatively spliced isoform of GFR{alpha}2. When activated by ligands (GDNF or NTN), GFR{alpha}2b inhibited neurite outgrowth induced by GFR{alpha}1a, GFR{alpha}2a, and GFR{alpha}2c isoforms. Furthermore, GFR{alpha}2b was found to inhibit a non-GFR{alpha} stimulus, retinoic acid-induced neurite outgrowth, and to activate RhoA.

Alternative splicing is prevalent in many mammalian genomes and is a means of producing functionally diverse polypeptides from a single gene (Blencowe, 2006Go). Recently, genome-wide microarray and large-scale computational analyses of expressed-sequence tag and cDNA sequences have estimated that >50% of human multi-exon genes are alternatively spliced (Modrek and Lee, 2002Go). Comparative genomic analyses also demonstrated that the greatest amount of conserved alternative splicing occurs in the CNS (Kan et al., 2005Go). In many systems, alternative splicing events have been shown to produce isoforms with distinct activities and biochemical properties, as a means for diverse biological functions (Lee and Irizarry, 2003Go).

In the cortex of human, mouse, and rat brain, the expression of GFR{alpha}2 mRNA has been reported (Sanicola et al., 1997Go; Widenfalk et al., 1997Go; Golden et al., 1998Go, 1999Go; Trupp et al., 1998Go). However, the probes used in these studies cannot distinguish the expressions of the isoforms. In the present study, we were able to specifically amplify all three isoforms in the human brain regions using exon-overlapping primers (Too, 2003Go). In the human brain, all three GFR{alpha}2 isoforms are expressed at comparable levels, with GFR{alpha}2c significantly higher than the other two isoforms. Compared with the other regions of the human brain, the cortex expressed the highest levels of the isoforms. The functional significance of these isoforms in the cortex has yet to be defined. Interestingly, the high expressions of the GFR{alpha}2 isoforms in the cortex, a region of the brain involved in learning complex tasks, and the observation that GFR{alpha}2 knock-out mice show significant impairment in several memory tasks (Voikar et al., 2004Go) may suggest a possible role of GFR{alpha}2 signaling in the development and/or maintenance of cognitive abilities that help in solving complex learning tasks.

GDNF and NTN are known to similarly activate a number of signaling pathways, including ERK, phosphatidylinositol 3-kinase/AKT, p38 MAPK, and JNK (Trupp et al., 1999Go; Takahashi, 2001Go; Pezeshki et al., 2003Go; Ichihara et al., 2004Go), and regulate the expressions of various immediate-early-response genes (Fukuda et al., 2003Go; Pezeshki et al., 2003Go). In this study, it is intriguing to note that the activation of specific signaling pathways but not the early-response genes is dependent on the ligands used. For instance, GDNF was found to potently activate ERK1/2 through GFR{alpha}2a and GFR{alpha}2c in a dose- and time-dependent manner but did not activate GFR{alpha}2b significantly. This was not attributable to the failure of GDNF to interact with GFR{alpha}2b because GDNF displaced bound [125I]GDNF equally well with all three isoform transfectants. Similarly, GDNF activated AKT through GFR{alpha}2b and GFR{alpha}2c but not through GFR{alpha}2a. However, NTN showed similar activations of ERK1/2 and AKT through all of the three isoforms. GDNF at lower concentrations appeared to be slightly more potent than NTN in the activation of ERK1/2 but not at higher concentrations in GFR{alpha}2a and GFR{alpha}2c transfectants. The significance of this, however, is unclear presently.

Both GDNF and NTN have previously been shown to have similar properties in activating the multicomponent receptor complex (Baloh et al., 1997Go; Airaksinen et al., 1999Go; Wang et al., 2000Go; Scott and Ibanez, 2001Go; Coulpier et al., 2002Go; Charlet-Berguerand et al., 2004Go). In addition, midbrain dopaminergic neurons that only express GFR{alpha}1 appear to survive equally well with both GDNF and NTN in vitro and in vivo (Horger et al., 1998Go). However, there are observations of distinct functional differences with the use of specific ligands. Although GDNF and NTN promote the survival of dopaminergic neurons through GFR{alpha}1 (Cacalano et al., 1998Go; Akerud et al., 1999Go), only GDNF possess neuritogenic and hypertrophic effects (Akerud et al., 1999Go). In cultured sympathetic neurons, GDNF was able to promote the survival of culture sympathetic neurons through GFR{alpha}2, but NTN could not promote survival through GFR{alpha}1 (Buj-Bello et al., 1997Go). Furthermore, GDNF but not NTN could promote the axonal growth of DRG neurons through GFR{alpha}1 (Paveliev et al., 2004Go). Consistent with these studies, recent observations show differential ligand signaling through the activation of GFR{alpha}1 (Lee et al., 2006Go) and distinct activation of microRNAs by specific ligands through the GFR{alpha}2 receptor complexes (Yoong et al., 2006Go), supporting the emerging view that cross talk of exogenously applied GDNF and NTN with a specific receptor may, in some instances, result in distinct functions.

It is well documented that GDNF and NTN are potent trophic factors that have potent effects on neuronal differentiation and promote survival and sprouting of ventral mesencephalic dopaminergic neurons in primary cultures and other neuronal cultures (Lin et al., 1993Go; Akerud et al., 1999Go; Baloh et al., 2000Go; Yan et al., 2003Go; Wanigasekara and Keast, 2005Go; Zihlmann et al., 2005Go). The finding in this study of a particular alternatively spliced variant of GFR{alpha}2 inhibiting neurite outgrowth was unexpected. Unlike GFR{alpha}2a and GFR{alpha}2c, GFR{alpha}2b transfectants did not induce neurite outgrowth when activated by either GDNF or NTN. Both GFR{alpha}2a and GFR{alpha}2c (but not GFR{alpha}2b) activated the early-response gene egr1 (also known as NGFI-A, krox-24, zif-268, and TIS-8), consistent with a role of egr1 in neuronal differentiation (Pignatelli et al., 1999Go; Knapska and Kaczmarek, 2004Go). In coexpression studies, GFR{alpha}2b was found to inhibit ligand-induced neurite outgrowth by GFR{alpha}2a and GFR{alpha}2c. Similarly, in BE(2)-C cells endogenously expressing GFR{alpha}2b isoform, both GDNF and NTN did not significantly alter the morphology of the cells. However, the silencing of GFR{alpha}2b and subsequent treatment with either GDNF or NTN caused the cells to extend neurite-like structures. Interestingly, in coexpression studies, fosB was upregulated and paralleled the upregulation of the immediate-response gene observed in GFR{alpha}2b transfectants by GDNF or NTN. It is not known whether the coexpression of GFR{alpha}2b with GFR{alpha}2a or GFR{alpha}2c may have affected the protein expression levels of the latter two spliced variants resulting in the attenuation of neurite extension on ligand stimulation. The possibility of GFR{alpha}2b affecting the expression of the other spliced variants and the effects of expressions levels is currently investigated.

The inhibition of neurite outgrowth by GFR{alpha}2b is not restricted to the GFR{alpha}2 family of isoforms. Ligand-activated GFR{alpha}2b also inhibited the neurite outgrowth induced by GFR{alpha}1a, another member of GFR. Intriguingly, the activation of GFR{alpha}2b inhibited neurite outgrowth induced by retinoic acid. The underlying GFR{alpha}2b inhibitory mechanism appears to involve the Rho family of GTPases. RhoA is a member of the Rho GTPase family, which includes RhoA, Rac, and Cdc42 (Luo, 2000Go; Van Aelst and Cline, 2004Go). Although Rac and Cdc42 have been shown to be involved in promoting neurite and axonal outgrowth, RhoA has been the focus in studies of molecular mechanisms for some glia-derived neurite outgrowth inhibitory factors such as Nogo-A, myelin-associated glycoprotein (Niederost et al., 2002Go), and LPA (Sayas et al., 2002Go). More recent findings have revealed that RhoA mediates neurite outgrowth inhibition by reorganization of actin and the microtubular network (Dickson, 2001Go; Leung et al., 2002Go). Consistent with these findings is that GDNF and NTN increased the active form of RhoA in GFR{alpha}2b but not GFR{alpha}2a or GFR{alpha}2c transfectants. Furthermore, the Clostridium botulinum C3 exoenzyme specifically ADP-ribosylates and inactivates Rho, thereby increasing neurite outgrowths in GFR{alpha}2a/GFR{alpha}2b and GFR{alpha}2c/GFR{alpha}2b coexpression models. It is interesting to note that GDNF induced RET-mediated phosphorylation of focal adhesion kinase, paxillin, and p130C through the activation of the Rho family of GTPase and inhibited the outgrowth of neurites in TGW-I-nu cells (Murakami et al., 1999Go). It is, however, unclear whether this observation is mediated through GFR{alpha}2b.

Compared with GFR{alpha}2a, both GFR{alpha}2b and GFR{alpha}2c showed deletion of eight cysteine residues and N-glycosylation sites at the N terminus (Wong and Too, 1998Go). GFR{alpha}2 is thought to be structurally organized into three distinct domains. The N-terminal domain has previously been shown to be dispensable for ligand binding specificity and RET phosphorylation of GFR{alpha} receptors (Scott and Ibanez, 2001Go). Extending this observation, the N-terminal domain encoding the unique sequences of GFR{alpha}2a, GFR{alpha}2b, and GFR{alpha}2c may serve to regulate distinct biochemical and cellular processes. It is tempting to speculate that the expression and interactions of specific GFR{alpha}2 receptor spliced isoforms may play an important role in neuronal differentiation involving GDNF and NTN. The recent observation in which the expressions of GFR{alpha}2 isoforms are differentially regulated in Nurr1-induced dorpaminergic differentiation of embryonic stem cells is consistent with this suggestion (Sonntag et al., 2004Go).

In summary, this study provides the first evidence that GDNF and NTN have distinct neuritogenic effects mediated through specific GFR{alpha}2 isoforms. GFR{alpha}2b inhibited GFR{alpha}1 and GFR{alpha}2, and retinoic acid mediated neuritogenesis through the Rho family of GTPases. The emerging view is that the combinatorial interactions of the spliced isoforms of GFR{alpha}2, RET, and NCAM may contribute to the complexity of a multicomponent signaling system and may produce the myriad of observed biological responses.


    Footnotes
 
Received Oct. 20, 2006; revised April 11, 2007; accepted April 14, 2007.

This work was partially supported by a grant from the Singapore–Massachusetts Institute of Technology Alliance. We thank Wan Guoqiang for his efforts in the development of the real-time PCR assays.

Correspondence should be addressed to Dr. Heng-Phon Too, Department of Biochemistry, National University of Singapore, Lower Kent Ridge Road, Singapore 119260. Email: bchtoohp{at}nus.edu.sg

Copyright © 2007 Society for Neuroscience 0270-6474/07/275603-12$15.00/0


    References
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

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