The Journal of Neuroscience, May 30, 2007, ():
Cell Cycle Regulator E2F4 Is Essential for the Development of the Ventral Telencephalon
J. Neurosci. Ruzhynsky et al.
27: 5926
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplementary figure 1. Expression of E2F4 in the developing mouse telencephalon. (A,B) Coronal sections through the telencephalon of E11.5 wild type mouse embryos were processed for in situ hybridization with E2F4 digoxigenin-labeled riboprobe. B – magnified image of indicated area in A (black box). Scale bars: A – 250 ?m; B – 50 ?m. (C) Analysis of E2F4 protein (~56 kDa) level in the proliferating neurospheres from wild type (WT) and E2F4-/- (KO) embryos by western blot. Non-specific band is indicated by asterisk. Actin was used as a loading control.
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Supplementary figure 2. E2F4 deficient embryos exhibit reduction of the developing striatal neuroepithilium and decreased expression of Lhx6 in the cortical marginal zone. In situ hybridization analysis of Lhx6 mRNA expression in E15.5 wild type (A,B) and E2F4 null (C,D) embryos. Note the reduced Lhx6 expression in the cortical marginal zone (MZ), as well as the smaller size of the striatum (st) and enlarged lateral ventricle (black asterisk). Scale bars: A, C – 250 ?m; B, D – 75 ?m.
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Supplementary figure 3. E2F4 deletion does not affect Shh expression in the notochord and spinal cord at E11.5. In situ hybridization analysis for Shh mRNA expression in the ventral spinal cord (A, SCv, black arrow) and notochord (A, NC, black arrowhead) of E11.5 wild type (WT) and E2F4-/- mouse embryos. Scale bar 250 µm.
