The Journal of Neuroscience, June 13, 2007, ():
Brain-Derived Neurotrophic Factor Regulates Cholesterol Metabolism for Synapse Development
J. Neurosci. Suzuki et al.
27: 6417
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Quantitative analysis of spontaneous mEPSCs of cultured hippocampal neurons treated with BDNF and/or mevastatin. Hippocampal neurons were cultured in the presence or absence of BDNF (200 ng/ml) and/or 10 µM mevastatin for 3 days. At DIV 6-7, spontaneous mEPSCs were measured. (A) Representative traces of spontaneous mEPSCs of the neurons treated with the indicated drugs. (B) Summary of the effect of BDNF and/or mevastatin on the frequency and amplitude of spontaneous mEPSCs. n= the number of independent cover slips.
- supplemental material
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Supplemental Figure 2. A time-course study of BDNF-induced activation of the TrkB receptor. Cultured cortical neurons were prepared and stimulated with BDNF (200 ng/ml) in the same manner as described in the legend to Figure 1. The cell lysates were collected at the indicated times after BDNF application and immunoblotted using antibodies against phospho-Trk (Y490) as described previously (Suzuki et al., 2004).
