The Journal of Neuroscience, June 13, 2007, ():
Corticotropin-Releasing Factor Receptors Differentially Regulate Stress-Induced Tau Phosphorylation
J. Neurosci. Rissman et al.
27: 6552
Supplemental Data
Files in this Data Supplement:
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Supplemental Fig. 1. Acute and repeated stress effects on PP2A-c levels. Western analysis of relative levels of PP2A-c in mice sacrificed 20 min or 24 hr after 30 min acute restraint or 14 consecutive daily exposures. Under control and acute stress conditions, low levels of free PP2A-c subunit were found within soluble fractions (lanes 1-3). Under conditions of repeated stress, PP2A-c protein was elevated at both time points (lanes 4-5). No PP2A-c signal was observed in detergent-soluble fractions (data not shown). These data implicate alterations in tau phosphatase activity as contributing to tau-P under repeated stress conditions, at least (cf Fig. 7). Data are expressed as mean ± SEM optical density. *, differs significantly from control, P<0.01; **, P<0.001. †, differs significantly from acute 20 min stress condition, P<0.01; †† P<0.001. ?-actin was used as a loading control.
