The Journal of Neuroscience, June 20, 2007, ():
Schwann Cell-Derived Factors Modulate Synaptic Activities at Developing Neuromuscular Synapses
J. Neurosci. Cao and Ko
27: 6712
Supplemental Data
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Cao and Ko, Supplemental Data
Figure S1. SC-CM contains high-temperature-sensitive factor(s).
We used heat-denature treatment to examine whether the active factor(s) in SC-CM were high temperature-sensitive proteins/peptides. SC-CM contained in a sealed centrifuge tube was immersed in the boiling water bath (100o C) for 20 minutes and then transferred to -20 o C freezer immediately for rapid cooling. Fig. S1 summarizes 4 studies comparing the effect of SC-CM before and after heat-denature treatment on SSC at the same neuromuscular synapses. The average increase in SSC frequency by heat-denatured SC-CM (filled diamond) is significantly lower than that by native SC-CM (from the same batch but without boiling, open square) (9.6 ± 1.3-fold vs. 253.7 ± 68.3-fold at 16 minutes after treatments). The residual increase in SSC frequency by heat-denatured SC-CM may be due to a partial reconstitution of these peptides following cooling. Although we cannot exclude the existence of a second type of small molecules that are not heat-sensitive, this study suggests that the major active factor(s) in SC-CM are likely heat-sensitive peptides.
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