The Journal of Neuroscience, June 20, 2007, ():
Specific Drosophila Dscam Juxtamembrane Variants Control Dendritic Elaboration and Axonal Arborization
J. Neurosci. Shi et al.
27: 6723
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Figure S1. Silencing of various UAS-transgenes by specific UAS-miRNAs
Composite confocal images of wandering larval (WL) MBs showing suppression of the GAL4-201Y-dependent induction of UAS-Dscam3.36.25.1::GFP and UAS-Dscam3.36.25.2::GFP by UAS-17.1 miRNA (C) and UAS-17.2 miRNA (F), respectively. In addition, UAS-18 miRNA antagonized the induction of both UAS-Dscam3.36.25.1::GFP (G) and UAS-Dscam3.36.25.2::GFP (H), but not UAS-mCD8::GFP (data not shown). Note that weak induction is necessary for demonstrating the preferential axon targeting of DscamTM2. For instance, the MB axon peduncle and lobes (e.g. arrowheads in F) are better labeled than the calyx when Dscam3.36.25.2::GFP was weakly expressed (F, H).
- supplemental material
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Figure S2. Requirement of DscamTM2 for EB axonal morphogenesis
Nb clones of adult EB neurons labeled with a flip-out reporter gene (A-C) or MARCM (D). Clones were generated in the absence of transgenic miRNA (A) or in the presence of UAS-17.1 miRNA (B) or UAS-17.2 miRNA (C). Note malformation of the EB ring following induction of UAS-17.2 miRNA (C). Similar phenotypes are observed in Dscam mutant clones (D).
