The Journal of Neuroscience, June 27, 2007, ():
A Subpopulation of Olfactory Bulb GABAergic Interneurons Is Derived from Emx1- and Dlx5/6-Expressing Progenitors
J. Neurosci. Kohwi et al.
27: 6878
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplement Figure 1 : CGE grafted to adult SVZ does not make OB cells
A, schematic of transplantation experiment; B, graft site; C, section of OB 40days after graft
Scale bar for B-C = 100|*mu*|m
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Supplemental Figure 2: Dlx5/6i-cre lineages in OB are GABAergic, but not vGlut1+ or vGlut2+
A/D, many EGFP+ cells express GABA at P0 and adult (arrows); B-C/E-F EGFP+ cells do not express vGlut1 or vGlut2 (arrowheads). Insets are confocal images at higher magnification.
Scale bar for A-F = 50µm
Scale bar for insets = 10µm
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Supplemental Figure 3: TBR1 is not expressed in the Dlx5/6i lineage nor in most Emx-1-lineage cells in the granule layer.
P0 OB. TBR1 immunofluorescence (red) with EGFP-expressing cells (green) from the Dlx5/6i lineage (left panel) and Emx1 lineage (right panel). Arrowheads show areas of non-overlap of TBR1 and EGFP in the Dlx5/6i lineage and Emx1 lineage in the glomerular and granule cell layers. Arrows point to overlap of TBR1 and EGFP in the Emx1 lineage in the mitral cell layer. Abbreviations: glom, glomerular layer; mcl, mitral cell layer; glc, granule cell layer.
Scale bar A, B = 50µm
Scale bar A', B' = 30µm
Scale bar A'', B''= 30µm
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Supplemental Figure 4: Emx1 lineage does not give rise to GABAergic cells in cortex, but Dlx5/6i lineage does.
Left panels show Emx1-cre lineage (green) and DLX2 (red) expression in the cortex at E15.5 and P0. Right panels from adult cortex show absence of DLX2 expression in the Emx1-cre lineage (top), GABA immunofluorescence (red) and EGFP (green) from Emx1-cre lineage (middle) and Dlx5/6i-cre lineage (bottom). Arrows point to non-overlap of GABA and EGFP in Emx1-cre lineage and coexpression of GABA with EGFP in the Dlx5/6i-cre lineage.
Scale bars = 50|*mu*|m
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Supplemental Figure 5: In situ RNA hybridization for Dlx1 (top row) and Emx1 (bottom row) on coronal E15.5 telencephalic sections of wildtype and Dlx1/2-/- mutant mice, arrayed from rostral (left) to caudal (right). See Long et al. (2007) for the molecular features of the cells in the pallial (P), subpallilal (SP), and mixed (M) progenitor domains. The mixed domain, which most closely resembles the molecular profile of the OB VZ, forms at the medial intersection of pallial and subpallial progenitor domains.
Emx1 expression in the wildtype (E-H) is found in glomerular and granule cell layers of the distal OB (E); in the caudal parts of the OB, Emx1 is expressed in the P, SP and M domains (F-G); whereas in the telencephalon proper its expression appears largely restricted to the P domain (H). Emx1 expression in the SP and M domains extends from the rostral confluence of the LGE and septum. In the Dlx1/2-/- mutant, Emx1 expression is decreased in the SP domains (large arrows) (F-F''), as well as in the rostral OB (E-E'), but not in the P or M domains. Note also, that In the Dlx1/2-/- mutant, there is persistent transcription of a truncated Dlx1 transcript that continues to mark the SP domain of proximal parts of the OB primordium: the truncated Dlx1 transcript is absent in the pallial (P) and mixed (M) domains (B-B', C-C') on the rostral OB (A-A'), suggesting the loss of Dlx1+ cells in these parts of the OB.
