The Journal of Neuroscience, June 27, 2007, ():
Striatal Medium Spiny Neurons Terminate in a Distinct Region in the Lateral Hypothalamic Area and Do Not Directly Innervate Orexin/Hypocretin- or Melanin-Concentrating Hormone-Containing Neurons
J. Neurosci. Sano and Yokoi
27: 6948
Supplemental Data
Files in this Data Supplement:
- supplemental material
-
Supplemental Figure 1. a, Fluorescent whole mount view of the GFP-expressing (PDE10A2-tTA/bitetO-GFP) mouse brain. The olfactory tubercle (OT) and the substantia nigra pars reticulata (SNr) are clearly visualized with green fluorescence in a bottom view (right). b, Coronal brain sections of GFP-expressing mice (PDE10A2-tTA/bitetO-GFP) visualized with green fluorescence. CPu, caudate-putamen; NAc, nucleus accumbens; OT, olfactory tubercle; VP, ventral pallidum; GP, globus pallidus; STh, subthalamic nucleus; SNr, substantia nigra pars reticulata; PPTg, pedunculopontine tegmental nucleus. c, High magnification of the CPu stained with red fluorescent Nissl solution in GFP-expressing mice (PDE10A2-tTA/bitetO-GFP). About 90 % of Nissl stained neurons show green fluorescence, suggesting that seemingly all MSNs express EGFP. Arrowheads: Nissl stained neurons without green fluorescence, looking like interneurons. Scale bars: 1 mm (b); 10 µm (c).
- supplemental material
-
Supplemental Figure 2. a,b, Immunostainings with anti-VGAT antibody (red) of the green fluorescently labeled terminal regions of striatal MSNs in GP (a) and SNr (b) of synaptopHluorin(spH)-expressing mice (PDE10A2-tTA/tetO-synaptopHluorin). Co-localization of spH and VGAT is observed. c, Fluorescent Nissl staining (red) of the anterior LHA in synaptopHluorin(spH)-expressing mice (PDE10A2-tTA/tetO-synaptopHluorin) shows that neurons were encircled within the green fluorescent plexus of the striatal MSN axons. Scale bars: 20 µm (a,b); 10 µm (c).
.gif){kind=link}
.gif){kind=link}
