The Journal of Neuroscience, June 27, 2007, ():
Critical Involvement of cAMP/DARPP-32 and Extracellular Signal-Regulated Protein Kinase Signaling in L-DOPA-Induced Dyskinesia
J. Neurosci. Santini et al.
27: 6995
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1 LID-associated increase in ERK phosphorylation occurs in striatal medium spiny neurons. Immunofluorescence detection of DARPP-32 (which is specifically expressed in medium spiny neurons) (a, d) and phosphoThr202/Tyr204-ERK (P-ERK) (b, e) in the dorsal striatum of a highly dyskinetic mouse. (c, f) Double immunolabeling showing co-localization of DARPP-32 and P-ERK. Scale bars 200 µm (a-c) and 40 µm (d-f).
- supplemental material
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Supplementary Figure 2 c-Fos immunoreactivity increases in dyskinetic mice. (a-d) Immunocytochemical detection of c-Fos in the dorsal striata of (a) sham-lesioned mouse (Sham), 6-OHDA-lesioned mouse with (b) low dyskinesia (L Dys) or (c) high dyskinesia (H Dys), (d) 6-OHDA-lesioned mouse treated with L-DOPA in combination with SL327 (75 mg/kg) (SL327). (e) 6-OHDA-lesioned mice were divided into low dyskinetic (L Dys) and highly dyskinetic (H Dys) according to their total AIMs score (see Methods) using the median value of 29 as cut-off. Histograms showing the number of c-Fos positive cells in the dorsal striata of sham-lesioned mice (Sham), L Dys, H Dys, and 6-OHDA-lesioned mice treated with L-DOPA in combination with SL327 (SL327). Data are means ± SEM (n=3-7). ***p<0.001 vs. L Dys, one-way ANOVA followed by Bonferroni-Dunn test. Scale bar (a-d) 40 µm.
