The Journal of Neuroscience, January 17, 2007, ():
Neurofilaments Switch between Distinct Mobile and Stationary States during Their Transport along Axons
J. Neurosci. Trivedi et al.
27: 507
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Movie 1. Pulse escape movie, 5 second intervals
Time-lapse movie showing a neurofilament departing from an activated region. The neurofilament moves in an anterograde direction in a rapid intermittent manner. The time elapsed following photoactivation is indicated in seconds. The images were acquired at 5 second intervals. Proximal is left, distal is right. Time compression=30:1.
- supplemental material
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Movie 2. Pulse-escape movie, 5 minute intervals
Time-lapse movie showing the pulse-escape behavior of an activated region over a period of two hours. Neurofilaments dispersed from the activated region in both anterograde and retrograde directions. The time elapsed following photoactivation is indicated in minutes. The movements of the individual filaments cannot be tracked because the images were acquired at 5 minute intervals. Note that some neurofilaments remained in the activated region even after two hours. Proximal is left, distal is right. Time compression=1800:1.
- supplemental material
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Movie 3. Photobleached gap animation
Flash™ animation illustrating the fluorescence photobleaching strategy for studying neurofilament movement in axons. Bleaching is used to create a gap in the fluorescence of the neurofilament array. Fluorescent neurofilaments that originate from elsewhere in the axon can be tracked for short periods of time as they move through the bleached gap. Note that with this technique neurofilaments can only be tracked if they move into the bleached gap; neurofilaments that remain paused on either side of the gap cannot be tracked because they cannot be resolved from their neighbors. Thus this approach underestimates the long-term pausing behavior of the neurofilaments.
- supplemental material
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Movie 4. Pulse-escape animation
Flash™ animation illustrating the pulse-escape fluorescence photoactivation strategy for studying neurofilament movement in axons. Photoactivation is used to label a population of neurofilaments in a short segment of axon. The movement of these fluorescent neurofilaments can be monitored by tracking their movement out of the activated region on a time scale of seconds or minutes, or by tracking the loss of fluorescence from the activated region on a time scale or minutes or hours. This approach can reveal both the short and long term pausing behavior of the neurofilaments.
