The Journal of Neuroscience, January 17, 2007, ():
Networks of Parvalbumin-Positive Interneurons in the Basolateral Amygdala
J. Neurosci. Woodruff and Sah
27: 553
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1.
EGFP expressing cells in the amygdala are parvalbumin positive. Panels in A show representative coronal sections of the BLA taken at approximately Bregma -3.6. Section on the left shows a typical slice from a wild type animal stained with the anti parvalbumin antibody and the section on the right shows a similar section from a parvalbumin-EGFP animal. B. Coexpression of EGFP and parvalbumin. C. Representative sections from the basal amygdala are shown at higher magnification. The panel on the left shows the distribution of EGFP positive neurons, the middle panel shows the same section stained for parvalbumin and the panel on the right shows a merged image. Note that cells that express EGFP but do not appear to express parvalbumin are evident. All cells positive for parvalbumin expressed EGFP. The drawing below is an illustration of the region of interest taken from the Paxinos and Watson Atlas at bragma -3.6 with the basal amygdala labelled (BL). Wildtype and transgenic animals were anaesthetised with pentobarbitone (50 mg/kg) and perfused transcardially with 4% paraformaldehyde. The brains were then removed and sectioned into 100µm slices and processed for parvalbumin immunohistochemistry as in the methods using Alexa Fluor -594 as the secondary antibody.
- supplemental material
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Supplementary Figure 2.
Morphologies of parvalbumin-EGFP positive interneurons. Shown are typical morphologies of four electrophysiological types of parvalbumin-EGFP positive interneurons. In each case neurons were recorded either with 2% neurobiotin (FS cell) or 100 µM Alexa 594 in the recording pipette. After recordings slices were fixed overnight in 4% paraformaldehyde. The next day slices were washed in PBS. Neurons loaded with Alexa 594 (AC, ST and DF neurons) were visualised on a Zeiss 510 confocal microscope and z-stacks constructed. Shown are two dimensional projections from the z-stack. For neurons filled with neurobiotin (FS) slices were soaked overnight in avidin-horseradish peroxidase (Vectastain ABC Elite Kit, Vector Laboratories Inc, CA USA). After washing in Tris buffer (0.1 M, pH 7.4) filled cells were visualised using the diaminobenzidine (DAB) procedure. All Slices were then mounted on albumin coated slides, dried overnight, dehydrated in an ascending series of alcohols, cleared in xylene and coverslipped in Permount.
