The Journal of Neuroscience, January 17, 2007, ():
Functional Heterogeneity of Retinal Dopaminergic Neurons Underlying Their Multiple Roles in Vision
J. Neurosci. Zhang et al.
27: 692
Supplemental Data
Files in this Data Supplement:
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Supplemental Methods
- supplemental material
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Supplemental Figure 1 - We performed recordings on ganglion cells that were labeled with DiI and then targeted for recording using imaging methods identical to those used for DA cell recordings. Light responses to 525 nm flashes (square waves below response traces) were recorded in 14/15 ganglion cells tested. We observed OFF responses in 4 ganglion cells (A), ON-OFF transient responses in 3 ganglion cells (B), and ON-sustained responses in 7 ganglion cells (C) indicating that both ON and OFF pathways were highly functional in our preparations. Application of 100 μM L-AP4 entirely blocked the ON-sustained responses of ganglion cells (Bottom trace in C, n= 6). Current scale bars: 40 pA.
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Supplemental Figure 2 – Light-responsive and light-independent DA cells responded identically to dopamine. Left panel: spontaneous spikes of light-responsive cells were reversibly inhibited by 50 μM dopamine (n = 3). Right panel: 50 μM dopamine also inhibited the spontaneous activity of light-independent DA cells (n = 3) indicating that light-independent cells were healthy.
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Supplemental Figure 3 – Neither L-AP4 (A), nor carbenoxelone (B) reveal light responses in light-independent DA neurons (525nm light). Similar results were obtained in 4 cells for L-AP4 application and 6 cells for carbenoxelone application. Flashes are indicated by square waves below response traces.
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Supplemental Figure 4 – Double label immunocytochemistry for DA cells (Anti-TH, red) and bipolar cells (Anti-CaBP5, green), reveals associations of DA cell processes (pointed by arrows) with bipolar cell terminals in the inner portion (ON-sublamina) of the IPL (Overlay). Scale bar = 20 μm.
