The Journal of Neuroscience, January 17, 2007, ():
Imaging Cerebral Gene Transcripts in Live Animals
J. Neurosci. Liu et al.
27: 713
Supplemental Data
Files in this Data Supplement:
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Supplemental Text
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Figure S1 shows the uptake and distribution of sODN-cfos-dig in the cerebellar folium and the olfactory bulb in one of four animals infused with sODN-cfos-dig (18 nmole with 0.1 ?g/ml lipofectin). Presence of sODN-dig one day after infusion was detected using FITC-IgG against dig (Cui et al. 1999). Tissue thickness was 20 ?m. Purkinjie neurons are located in the Purkinjie layer as aligned stacking cells with a large nucleus (A).
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Figures S2 and S3 show FITC-sODN-biotin (100 pmol/?l) after conjugation with freshly prepared SPION-NA (270 pmol SPION per lane) before purification. Photographs show the gel at two time points (S1 = 200 and S2 = 1500 volt-hours) after electrophoresis under a 302/365 nm light source (exposure time 1/8 second for Fig S2, and 8 seconds for Fig S3). The distance of elution from the well for each gel slice is shown in centimeters (cm). The top row of each panel shows the concentrations of components in each sample applied to the agarose gel.
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Figure S4 shows FITC-sODN after electrophoresis (300 volt-hours, 150 volts per hour, exposure time 8 seconds). Purified SPION-NA was mixed with FITC-sODN-biotin before loading to the well. Two batches of SPION-NA preparations (A = 17 months and B = 2 months self lives) are shown at four different time points following preparation. Preparation within 6 months is considered fresh. Signal at 8 and 13 cm appears across the gel even in lanes where no FITC was applied (lane 6–8); the origins are not known but they are tailing the dye marker (short arrows).
