The Journal of Neuroscience, July 25, 2007, ():
Protracted Synaptogenesis after Activity-Dependent Spinogenesis in Hippocampal Neurons
J. Neurosci. Nägerl et al.
27: 8149
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. TBS activity induces the growth of persisting spines. List of all newly-formed spines that were observed during the extended (and not the short-term) timelapse imaging experiments (between 19-24 hours), in an arbitrary order, the bars indicating the maximal (black) and minimal (grey) age range constrained by the variable timelapse intervals; ‘fix’ means that the spine was present in the last image stack before fixation. The TBS was applied at one hour, indicated by the red arrow.
- supplemental material
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Supplemental Figure 2. Further examples of electronmicrographs. A1 and A2 show two examples of labeled control spines (s) being contacted by vesicle-filled boutons (b) via a synaptic cleft (white arrow head). B shows an example of newly formed young spine from the experiment shown in Figure 3 and that also does not form a synapse with the bouton (in blue) that it is in contact with. However, this bouton forms a synapse with an unlabeled target as indicated in the reconstruction in B3, the red region indicating the site of the synapse on that bouton. C shows two examples of older spines (s). The arrow heads point at the synaptic cleft of asymmetric synapses between the labeled spines (s) and the boutons (b).
