The Journal of Neuroscience, August 29, 2007, ():
Seizure-Associated, Aberrant Neurogenesis in Adult Rats Characterized with Retrovirus-Mediated Cell Labeling
J. Neurosci. Jessberger et al.
27: 9400
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1. Ectopic migration of newborn granule cells into the hilus.
DCX-expressing, newborn immature neurons in control rats are largely confined to the granule cell layer and SGZ (A). Three (B) and 5 weeks (C) following KA-induced SE a substantial number of DCX-expressing cells have morphological features that suggest ectopic, chain-like migration towards the hilus. The dotted yellow line depicts the border of the granule cell layer. Scale bar: 50 µm. ML: molecular layer, GCL: granule cell layer.
- supplemental material
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Supplementary Figure 2. Aberrant neurogenesis in male rats following KA-induced SE.
Retroviral labeling of newborn granule cells in male controls (A) and animals that had seizures (B, C) revealed morphological alterations very similar to female rats (see Fig. 1). Four weeks after viral injection, newborn cells under control conditions extended an apical dendrite that was covered with dendritic spines towards the ML (A). In sharp contrast and reflecting the situation in female rats, KA-induced SE results in the formation of aberrant newborn neurons extending basal dendrites deep into the hilus (B) or being ectopically localized into the hilus (C), which was never observed in control animals. Arrows indicate apical dendrites; arrowheads label basal dendrites. Pan-neuronal marker NeuN is red in all images. Small panels in A, B show the GFP signal alone (upper panel) and a high power view of newborn cells (lower panel). Scale bars: 50 µm. ML: molecular layer, GCL: granule cell layer.
