The Journal of Neuroscience, September 12, 2007, ():
Synaptic Integration of Adult-Generated Olfactory Bulb Granule Cells: Basal Axodendritic Centrifugal Input Precedes Apical Dendrodendritic Local Circuits
J. Neurosci. Whitman and Greer
27: 9951
Supplemental Data
Files in this Data Supplement:
- supplemental material
-
Figure S1: Adult-generated cells express bassoon, a presynaptic marker.
Bassoon (red) puncta in the EPL first colocalize with spine heads on labeled cells (green) at 21 dpi. Images on the left are maximum projections of confocal images, and images on the right are higher magnification, single optical plane, of the boxed areas on the left, showing the z dimension. Scale bar in B = 10?m.
- supplemental material
-
Figure S2: The alpha 1 subunit of the GABA A receptor is apposed to labeled spines.
GABA A receptor alpha 1 subunit (red) puncta are adjacent and apposed to spine heads on labeled cells (green) at 21 dpi, labeling the postsynaptic mitral/tufted dendrite receiving the synapse from the GFP-labeled spines. Images on the left are maximum projections of confocal images, and images on the right are higher magnification, single optical plane, of the boxed areas on the left, showing the z dimension. Scale bar = 10?m.
- supplemental material
-
Figure S3: Model of synaptic integration in the GCL.
As soon as 10 dpi in the SVZ, new cells finish migrating and reach their place in the GCL, extend an apical dendrite towards but not into the EPL, and receive synapses at the cell body and basal dendrites from centrifugal fibers. At 14 dpi, the apical dendrite branches within the EPL and grows dendritic spines, which are present for up to a week before they develop reciprocal synapses with the dendrites of mitral tufted cells at 21 dpi. Spine density in the EPL increases to 42 dpi, near the end of the major period of cell death, before decreasing again by 56 dpi. New cells thus receive input from the circuit into which they are integrating before they are able to make any output.
