The Journal of Neuroscience, September 26, 2007, ():
Progranulin Mediates Caspase-Dependent Cleavage of TAR DNA Binding Protein-43
J. Neurosci. Zhang et al.
27: 10530
Supplemental Data
Files in this Data Supplement:
- supplemental material
-
S1. Progranulin is present in cytoplasm of most HeLa cells (with less nuclear localization) with anti-progranulin immunohistochemistry.
- supplemental material
-
S2. Biochemical analyses of TDP-43 in sporadic and familial (Gly333ValfsX28 PGRN mutation) FTLD-U (Gass et al., 2006) AD and vascular dementia (VaD) brains. Immunoblots of Triton-X soluble (Sol) and Triton-X insoluble (Ins) fractions from temporal cortex of cases and controls with rabbit anti-TDP-43 antibody shows a similar biochemical profile of TDP-43 to that observed in progranulin knockdown experiments in cultured cells (Figure 1B and 2A).
- supplemental material
-
S3. Histone immunocytochemistry demonstrates mostly nuclear staining in H4 cell treated with 1 µM staurosporine, demonstrating that the TDP-43 subcellular redistribution is unlikely to be related to nonspecific leakage of DNA binding proteins from nucleus to cytoplasm induced by apoptosis.
