Figure 4.
Cloning and expression of olrn-1 gene and cell-specific rescue of the olrn-1(ut305) butanone enhancement phenotype. A, Genetic map, physical map, and genomic rescue experiments. The bottom indicates the regions of the genome used for rescue experiments as well as the results of rescue experiments. B, Predicted amino acid sequence of OLRN-1 (a- and b-type). Predicted transmembrane domains and the region showing homology to Drosophila Raw are shown, together with a partial amino acid sequence of Raw. C, Rescue of the butanone (But) enhancement defect of olrn-1 with the wild type olrn-1 cDNA driven by a variety of cell-specific promoters. The selection assay was used to detect the rescue sensitively. The left two columns show control experiments with wild-type and olrn-1(ut305) animals. The olrn-1 cDNA driven by the H20 (pan-neuronal), gcy-10 (AWC, AWB, and I1), and odr-3 (AWC, AWA, AWB, ASH, and ADF) promoters, but not str-1 (AWB), str-2 (AWCON), or ttx-3 (AIY) promoters, rescued all of the olrn-1 mutant phenotypes. Four to six plates were used for all data. *p < 0.005, comparing the olrn-1 mutant with the olrn-1 mutant carrying transgenes. The error bars indicate SEM. Iso, Isoamyl alcohol.