The Journal of Neuroscience, October 10, 2007, ():
Real-Time Imaging of Discrete Exocytic Events Mediating Surface Delivery of AMPA Receptors
J. Neurosci. Yudowski et al.
27: 11112
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Example of an SEP-AMPAR insertion event (arrow) quenched by surface acidification. 50 mM MES pH5.25 was added to the culture medium at 0.3 sec. Essentially complete quenching (>90% reduction in fluorescence intensity) was observed in all examples. The sequence shown is representative of 3 independent experiments. Scale bar is indicated in the lower left corner. Time stamps (in sec) are indicated in the lower right corner of each frame.
- supplemental material
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Supplemental Movie 1. Rapid time-lapse series (100 msec / frame) showing SEP-AMPAR fluorescence on the cell body and proximal dendrites of a pyramidal neuron imaged in a cultured slice under basal conditions. SEP-AMPAR insertion events are evident as bright puffs of localized surface fluorescence that appear within a single frame. Fluorescence intensity measurement of the indicated region, including a transient puff, is plotted below. Each plane corresponds to a single 100 msec frame of sequential acquisition.
- supplemental material
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Supplemental Movie 2. Rapid time-lapse series of a region of a distal dendrite imaged in the cultured slice preparation, showing an example of a persistent SEP-AMPAR insertion event.
- supplemental material
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Supplemental Movie 3. Rapid time-lapse series (100 msec / frame) of the cell body of a representative pyramidal neuron imaged by TIR-FM in dissociated neuronal culture showing multiple SEP-AMPAR insertion events.
- supplemental material
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Supplemental Movie 4. Rapid time-lapse series (100 msec / frame) of a dissociated culture showing a wide-field image of dendrites, in which numerous SEP-AMPAR insertion events are observed.
- supplemental material
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Supplemental Movie 5. SEP-AMPAR insertion events imaged at higher magnification in dendrites. The field was imaged in the wide field at 100 msec / frame, and corresponds to that shown in Fig 3. Localization of endogenous PSD-95, determined by post-hoc immunostaining, is overlaid in red.
