The Journal of Neuroscience, November 7, 2007, ():
Activity-Dependent Synaptic Plasticity in Retinal Ganglion Cells
J. Neurosci. Xia et al.
27: 12221
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1. AMPA-elicited currents in cultured retinal neurons were measured during PhTX application to block GluR2-lacking AMPARs. Graph depicts the average normalized AMPAR currents in cells grown overnight in the presence of CNQX. PhTX causes a similar blockade of AMPAR-currents as NASPM in CNQX treated neurons (n=10). Inset: Traces of AMPA currents from representative cells before (black) and after (grey) the application of PhTX.
- supplemental material
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Supplemental Figure 2. Model for activity-dependent regulation of synaptic AMPAR composition in ON ganglion cells. In the absence of synaptic activity associated with darkness (top), GluR2-containing, but not GluR2-lacking, AMPARs cycle into and out of the extrasynaptic membrane. A proportion of the GluR2-containing AMPARs are bound to PICK1 or GRIP preventing their rapid diffusion into the synaptic membrane. In the light (bottom), the cycling of AMPARs is inhibited and GluR2-containing AMPARs from the cycling pool localize to the synaptic membrane, increasing the proportion of these receptors. The GluR2-containing receptors are not influenced by their interaction with PICK1/ GRIP and are able to diffuse freely into the synapse, thereby altering the composition of synaptic AMPARs.
