The Journal of Neuroscience, January 31, 2007, ():
Munc13-1 C1 Domain Activation Lowers the Energy Barrier for Synaptic Vesicle Fusion
J. Neurosci. Basu et al.
27: 1200
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1.
Recovery of RRP after depletion is faster in PDBu:
Upper panel, Responses of excitatory wild type neurons to 4s pulses of 500mM sucrose applied consecutively at varying intervals of time in the absence (dark blue) and presence (light blue) of 1µM PDBu. Lower, Plot showing the mean fraction of the RRP to be released at varying intervals of time (1 s, 3 s, 5 s and 7 s) after complete depletion of the pool. PDBu increases the rate at which vesicles are released after depletion of pool signifying that in the activated state of Munc13 that a greater fraction of vesicles can reach the energy threshold for fusion faster as against the naïve ‘inhibited’ state.
- supplemental material
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Supplementary Figure 2.
C1 domain activation modulates short-term plasticity: Upper panel, Raw traces showing paired pulse responses from naïve (dark blue) and PDBu treated (light blue) excitatory wildtype neurons at varying inter pulse intervals of 20, 40 and 100 ms. Cells that tend to facilitate in response to higher frequency stimulations (IPI, 20-40 ms) show chronic depression in PDBu under the same paired pulse paradigm. Lower, plot showing the mean paired pulse ratio and how it varies with C1 domain activation (n=29).
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