The Journal of Neuroscience, February 7, 2007, ():
Differential Spatial Representation of Taste Modalities in the Rat Gustatory Cortex
J. Neurosci. Accolla et al.
27: 1396
Supplemental Data
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Supplemental material
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Figure S1. Topographical Representation of Tastant Modalities Using Linear Model Based Statistics. A, Schematic description of the linear model (LM) for intrinsic signal. On the left a typical time-course of the signal is shown, taken from the pixel in the activated region (frame below). An exponential fitting is showed in superposition (dashed red lines). Fitted decay time constant is 3s. The signal could be decomposed into the sum of an exponential decay (middle) and a residual (right). B, Population maps for the four basic taste modalities NaCl (n = 18), sucrose (n = 15), citric acid (n = 8) and quinine (n = 8). Imaging was done on a total of 27 animals, testing at least two tastants chosen randomly. Color scale Min = 20 %, Max = 100% of the total number of rats. C, Matrices showing the relative degree of overlap (° Ovl) between stimuli. The three matrices are computed from different consistency indices (30, 50 and 70%). Color coded values are the result of the ratio between the overlap area (yellow in C) and the total activated area. Value for white squares is 1. D, Overlap curves as a function of the threshold of animals responding. Mean overlap values among same tastants pairs (black) and different tastants pairs (red). Difference between black and red line is statistically significant using ANOVA (stars: Newman-Keuls post hoc test, at least p < 0.04).
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Figure S2. Fluid localization in the oral cavity and responses in absence of Chorda Tympani (CT) nerve. A, B, Parts of the oral cavity reached by a blue coloured solution (Methylene Blue diluted in distilled water). The fluid was delivered through our stimulation system at the end of an imaging experiment with the same stimulation protocol and in the same position used for imaging. Fungiform papillae (FP) and part of the Foliate (Fol) are stained (A) as well as the palate (B), whereas the circumvallate (CV) at the back of the tongue is not reached. C, Two different examples (2 different rats) of activation maps following sucrose (Top row) and quinine (Bottom) stimulation, both in the intact animal (middle) and in the same animal with bilateral CT nerve cut (right). Little or no response is left after the nerve impairment. D, Histograms representing the average responses across n = 3 animals before and after the CT nerve cut. No response on average is left after CT nerve cut (green column). The difference of the response in the same area, before and after is statistically significant (p < 0.05).
