The Journal of Neuroscience, February 21, 2007, ():
Neuroprotective Effects of Regulators of the Glycogen Synthase Kinase-3ß Signaling Pathway in a Transgenic Model of Alzheimer's Disease Are Associated with Reduced Amyloid Precursor Protein Phosphorylation
J. Neurosci. Rockenstein et al.
27: 1981
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Complementary behavioral data in the water maze for hAPP tg mice treated with lithium or crossed with DN-GSK3β tg mice. A, Testing time to reach the platform during the hidden portion of the water maze assay. Saline-treated hAPP tg mice (6 months old) showed performance deficits compared to the nontg saline-treated mice that were ameliorated by treatment with lithium. *p < 0.05 compared to saline-treated hAPP tg mice by one-way ANOVA with post-hoc Tukey-Kramer; n = 6 mice per group. B, Probe test that determines the number of entrances and time spent in the target quadrant did not show significant differences among the three groups. C, Testing time to reach the platform during the hidden portion of the water maze assay. hAPP tg mice showed performance deficits compared to the nontg and DN-GSK3β tg mice that were rescued in hAPP tg/DN-GSK3β tg animals (6 months old). *p < 0.05 compared to hAPP tg mice by one-way ANOVA with post-hoc Tukey-Kramer; n = 6 mice per group. D, Probe test that determines the number of entrances and time spent in the target quadrant did not show significant differences among the four groups.
- supplemental material
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Supplemental Figure 2. Complementary behavioral data in the water maze and biochemical data for nontg mice treated with lithium. A, Distance to reach the platform during the hidden portion of the water maze assay. Saline- and lithium-treated nontg mice (6 months old, n = 6 mice per group) showed comparable performance abilities in this test. B, Probe test that determines the number of entrances and time spent in the target quadrant did not show significant differences between the two groups. C, Representative Western blots demonstrating the patterns of immunoreactivity of full-length (FL) APP and phosphorylated forms of FL APP (FL APP-p) in homogenates from the frontal cortex of 6-month-old saline- and lithium-treated nontg mice. D, Image analysis of the levels of FL APP and FL APP-p in the frontal cortex of saline- and lithium-treated nontg mice. *p < 0.05 compared to saline-treated nontg mice by two-tailed unpaired Student’s t-test; n = 6 mice per group.
