The Journal of Neuroscience, February 21, 2007, ():
Deletion of Nf1 in Neurons Induces Increased Axon Collateral Branching after Dorsal Root Injury
J. Neurosci. Romero et al.
27: 2124
Supplemental Data
Files in this Data Supplement:
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Supplemental data No. 1. Embryonic control DRG neurons treated with (A) NGF or (B) NT-3 show non-branched and branched morphology, respectively. (C) In absence of neurotrophic support, Nf1SynIKO neurons display a highly branched morphology, similar to that of NT-3 treated control neurons, and (D) are significantly increased in number (237 ± 82; n=5) compared to their control counterparts (56 ± 9; n=8). Scale bar = 250μm. (* = P< 0.0001). One way ANOVA. Data represents mean ± s.e.m.
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Supplemental data No. 2. Videorecording of an Nf1SynIKO mouse at one week after unilateral dorsal rhizotomy (cut injury).
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Supplemental data No. 3. Videorecording of an Nf1SynIKO mouse at four weeks after unilateral dorsal rhizotomy (cut injury).
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Supplemental data No. 4. Videorecording of a WT control at four weeks after unilateral dorsal rhizotomy (crushed injury).
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Supplemental data No. 5. Videorecording of a Smad3-/- control at four weeks after unilateral dorsal rhizotomy (crushed injury).
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Supplemental data No. 6. Lack of Regenerative Advantage in Nf1-Deficient Neurons 30 days After Injury. Immunofluorescence visualization of NF-200 (A, D, G), GFAP (B, E, H), and NF-200/GFAP (C, F, I) at the DREZ of sham operated (large arrows; A-B), injured control (arrowheads; D-E) and rhizotomized Nf1SynIKO (arrowheads; G-H) mice. NF-200-positive fibers in the dorsal columns of sham-operated controls (asterisk in A) are absent in both control and Nf1 null animals after injury (asterisks in D, G), indicating failure to regenerate. Small arrows in D, G show axons in the dorsal roots. All injured animals show similar astrocytic scaring (arrowheads; E, H). Scale bar = 50 μm.
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Supplemental data No. 7. Cell viability of spinal cord slices in culture. After 48 hrs in culture, Propidium iodide (PI) is added 1hr prior to imaging. Minimal fluorescence from dead cells is observed in slices of either wild type (A) or Nf1SynIKO (B) spinal cords, compared to that induced after another 48 hr cooling at 4°C (C, D). The cell death index is reported as the fluorescent intensity at 1hr divided by that recorded after 48hr at 4°C. No statistical difference in cell death index is observed between WT and Nf1SynIKO slices (E). Scale bar = 250 uM. Student’s t-test. Numerical values are expressed in the figures as percentage of control. At least 10 slices from 2 different animals of each genotype are included.
