Figure 1.
Cre-mediated inactivation of Nf1 in proprioceptive DRG neurons. A–C, Colabeling of TrkC (A) and LacZ (B) in thoracic and lumbar DRG neurons in adult SynICre/Rosa26 mice, reveals that the majority of LacZ+ cells are TrkC+, and thus proprioceptive in nature (C; merged image). D, Quantification of 1016 DRG neurons from three different ganglia shows that 29.2 ± 5.8% of them are LacZ+, and thus undergo Cre-mediated recombination. E, F, Size distribution analysis of LacZ+ cells indicates that the majority of the recombined cells are the size of proprioceptive neurons (>22 μm in diameter). G, H, Colocalization of LacZ with NF200 (Ia proprioceptive cells) (H, arrows), but not with CGRP (C-type nocioceptive neurons) (G, arrows) further confirms the proprioceptive nature of most Cre-recombined DRG neurons. Scale bars: E, 50 μm; G, 200 μm; G, inset, 50 μm.