The Journal of Neuroscience, February 28, 2007, ():
Dual Role for LIM-Homeodomain Gene Lhx2 in the Formation of the Lateral Olfactory Tract
J. Neurosci. Saha et al.
27: 2290
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Supplementary figure
OB interneurons are generated in the lateral ganglionic eminence (LGE), from where they migrate towards the OB. Dopaminergic periglomerular interneurons as well as GABAergic interneurons of the granule cell layer follow this route (Betarbet et al., 1996). In control brains, GAD67-positive interneurons have yet to enter the out-pouching of the wild-type OB that is marked by AP2ε expression (a-c). In the Lhx2 mutant, AP2ε expression is offset to one side (dorsal and lateral) of the GAD67 staining. In (parassagittal) lateral sections (d-f) AP2ε staining covers the largest territory while GAD67 staining is quite distant. At intermediate levels (g-I), the tip of a thin GAD67 positive stream has reached the AP2ε- positive Lhx2 mutant OBLS. In most medial sections (j-l) only GAD67 expression is seen. Therefore the OBLS explants in the explant culture assay are likely to have projection neurons as the dominant population, with a small number of interneurons on one side of the explant. Arrows (a,d,g) indicate AP2ε expression and arrowheads (b,h,k) indicate the GAD67expression. c,f,i,l are overlays of a,b; d,e; g,h; and j,k respectively.
