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The Journal of Neuroscience, February 28, 2007, 27(9)
This Week in The Journal
This Week in The Journal
Cellular/Molecular
Neurosteroids and Tonic GABAergic Inhibition
Jamie Maguire and Istvan Mody
(see pages 2155–2162)
Fluctuations in progesterone levels during the ovarian cycle can alter the subunit composition of GABAA receptors. In this week's Journal, Maguire and Mody link this effect to neurosteroid production. The authors focused on the
subunit that underlies GABA-mediated tonic inhibition of dentate granule cells in the hippocampus. The progesterone receptor antagonist RU486 had no effect on GABAA subunit expression in cycling mice. However, finasteride, which inhibits synthesis of neurosteroids from progesterone, prevented upregulation of the
,5
Development/Plasticity/Repair
Engineering Astrocytes as Injury Repairmen
William B. J. Cafferty, Shih-Hung Yang, Philip J. Duffy, Shuxin Li, and Stephen M. Strittmatter
(see pages 2176–2185)
Unfortunately, the CNS throws up a number of roadblocks to axonal regeneration. One of these, chondroitin sulfate proteoglycans (CSPGs), is a major component of the glial scar that forms at the site of a CNS injury. This week, Cafferty et al. exploit the bacterial enzyme chondroitinase ABC (ChABC), which can digest CSPGs, to reopen the roadway. The authors made a transgenic mouse that expressed ChABC in astrocytes under the control of the gfap promoter. After dorsal hemisection of the spinal cord, corticospinal tract axons in wild-type mice stopped abruptly rostral to the lesion. In the transgenic mice, however, axons extended into the lesion, but motor function was not improved. However, after dorsal rhizotomy, axons of sensory neurons grew back through the dorsal root entry zone and into the CNS and also improved sensory function. The next CNS regeneration strategy may be to combine ChABC with targeting of myelin-associated inhibitors.
Behavioral/Systems/Cognitive
The Homunculus, Up Close and Personal
Kai J. Miller, Eric C. Leuthardt, Gerwin Schalk, Rajesh P. N. Rao, Nicholas R. Anderson, Daniel W. Moran, John W. Miller, and Jeffrey G. Ojemann
(see pages 2424–2432)
When it comes to recording of cortical activity, the closer one is to the source, the better the information. This week, Miller et al. used subdural grid electrodes over sensorimotor cortex to examine electrocorticographic (ECoG) signals associated with motor movements. Their subjects were patients awaiting epilepsy surgery who had intracranial electrode arrays in place to assist seizure localization. Voluntary movements of the tongue, hand, or other body parts produced spectral shifts in areas corresponding to the classical homunculus. The authors used the data from 22 subjects to create maps of cortical activity in different frequency ranges. In the low-frequency band (LFB) (8–32 Hz), movements reduced the signal amplitudes at 20–30 Hz, whereas in the high-frequency band (HFB) (76–100 Hz), movements increased the EcoG. The HFB signals represented activity that was more local than the LFB signals. The high fidelity of the EcoG may make it a useful complement to other methods of functional brain mapping.
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A grid of subdural electrodes (white dots) can be seen on a lateral skull x ray in a patient undergoing evaluation for epilepsy surgery. The grid was placed over the sensorimotor cortex as shown with standardized brain template. See the article by Miller et al. for details.
Neurobiology of Disease
A Forward Genetic Screen in Mice for Recessive Deafness
Martin Schwander, Anna Sczaniecka, Nicolas Grillet, Janice S. Bailey, Matthew Avenarius, Hossein Najmabadi, Brian M. Steffy, Glenn C. Federe, Erica A. Lagler, Raheleh Banan, Rudy Hice, Laura Grabowski, Elisabeth M. Keithley, Allen F. Ryan, Gary D. Housley, Tim Wiltshire, Richard J. H. Smith, Lisa M. Tarantino, and Ulrich Müller
(see pages 2163–2175)
This week, Schwander et al. take a step forward in the pursuit of recessive genes for deafness. The authors undertook a forward genetics screen by exposing male mice to the mutagen ENU (N-ethyl-N-nitrosourea) and then breeding them with wild-type females. Fifty-one of 850 pedigrees analyzed had impaired acoustic startle response, and 19 of the 51 had abnormal auditory thresholds. Fourteen of 19 lines showed recessive inheritance patterns. The authors have thus far identified hair cell defects attributable to point mutations in four genes, all of which have been linked to deafness in humans. Human missense mutations in one gene, pejvakin produce a nonprogressive auditory neuropathy as a result of defects in auditory nerve cells. In contrast, the mutation identified in this screen introduced a stop codon in pejvakin. This mutation resulted in progressive hearing loss caused by outer hair cell malfunction, suggesting distinct functions of this gene in neurons and hair cells.
Related articles in J. Neurosci.:
- Neurosteroid Synthesis-Mediated Regulation of GABAA Receptors: Relevance to the Ovarian Cycle and Stress
- Jamie Maguire and Istvan Mody
J. Neurosci. 2007 27: 2155-2162.[Abstract] [Full Text] - A Forward Genetics Screen in Mice Identifies Recessive Deafness Traits and Reveals That Pejvakin Is Essential for Outer Hair Cell Function
- Martin Schwander, Anna Sczaniecka, Nicolas Grillet, Janice S. Bailey, Matthew Avenarius, Hossein Najmabadi, Brian M. Steffy, Glenn C. Federe, Erica A. Lagler, Raheleh Banan, Rudy Hice, Laura Grabowski-Boase, Elisabeth M. Keithley, Allen F. Ryan, Gary D. Housley, Tim Wiltshire, Richard J. H. Smith, Lisa M. Tarantino, and Ulrich Müller
J. Neurosci. 2007 27: 2163-2175.[Abstract] [Full Text] - Functional Axonal Regeneration through Astrocytic Scar Genetically Modified to Digest Chondroitin Sulfate Proteoglycans
- William B. J. Cafferty, Shih-Hung Yang, Philip J. Duffy, Shuxin Li, and Stephen M. Strittmatter
J. Neurosci. 2007 27: 2176-2185.[Abstract] [Full Text] - Spectral Changes in Cortical Surface Potentials during Motor Movement
- Kai J. Miller, Eric C. Leuthardt, Gerwin Schalk, Rajesh P. N. Rao, Nicholas R. Anderson, Daniel W. Moran, John W. Miller, and Jeffrey G. Ojemann
J. Neurosci. 2007 27: 2424-2432.[Abstract] [Full Text]
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