Figure 7.
The importance of Ser-137 of WAVE2 in PDGF-induced migration of reaggregated OPCs. A, After stimulation with PDGF for 0–1440 min, OPCs were lysed, immunoprecipitated with an anti-phosphorylated Cdk substrate antibody, and immunoblotted with an anti-WAVE2 antibody (H-110). The cell lysates were also immunoblotted with an anti-WAVE2 or β-actin antibody. B, C, OPCs were pretreated with or without 10 μm PP1 (B) or 10 μm roscovitine (C). After incubation with PDGF for 360 min, serine phosphorylation of WAVE2 was assayed. D, E, OPCs were infected with the retro-IRES, wild-type WAVE2, WAVE2 S137A, or WAVE2 S137E. At 16 h after stimulation with PDGF, reaggregates were fixed with paraformaldehyde, blocked, and stained with an anti-ZsGreen antibody (green). Data were evaluated using Student's t test (*p < 0.01). IP, Immunoprecipitate.