Abstract
Calcium homeostasis plays a major role in maintaining neuronal function under physiological conditions. Amyloid-β (Aβ) initiates pathological processes that include disruption in intracellular calcium levels, so amelioration of the calcium alteration could serve as an indirect functional indicator of treatment efficacy. Therefore, calcium dynamics were used as a measure of functional outcome. We evaluated the effects of the anti-Aβ antibody aducanumab on calcium homeostasis and plaque clearance in aged Tg2576 mice with in vivo multiphoton imaging. Acute topical application of aducanumab to the brain resulted in clearance of amyloid plaques. Although chronic systemic administration of aducanumab in 22-month-old mice did not clear existing plaques, calcium overload was ameliorated over time. Therefore, this antibody likely restores neuronal network function that possibly underlies cognitive deficits, indicating promise as a clinical treatment. In addition, functional readouts such as calcium overload may be a more useful outcome measure to monitor treatment efficacy in models of Alzheimer's disease compared with amyloid burden alone.
SIGNIFICANCE STATEMENT Alzheimer's disease (AD) is a progressive neurodegenerative disorder that is currently without a cure. Aducanumab is an anti-amyloid-β antibody being developed for the treatment of AD. Interim analyses of a phase 1b clinical trial have suggested potential beneficial effects on amyloid pathology and cognitive status in patients treated with aducanumab (Sevigny et al., 2016). Here, we show that a murine analog of aducanumab clears amyloid plaques in an acute setting and restores calcium homeostasis disrupted in a mouse model of AD upon chronic treatment. Therefore, we demonstrate that aducanumab reverses a functional outcome measure reflective of neural network activity.
Introduction
Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by the presence of amyloid plaques and neurofibrillary tangles. Transgenic mouse models overexpressing amyloid precursor protein (APP) and developing amyloid-related pathologies over time are valuable systems to investigate amyloid-targeted approaches for the treatment of AD. Anti-amyloid-β (Aβ) immunotherapy has shown the ability to prevent the formation of pathology in mouse models (Bard et al., 2000, Dodart et al., 2002, Bard et al., 2003, Wisniewski and Goni, 2014) and there is tremendous promise in this approach to prevent, if not reverse, the progression of AD in humans. Amyloid plaque clearance has been one of the primary end points of therapeutic efficacy in most preclinical studies but often has not translated into therapeutic efficacy in clinical trials (Doody et al., 2014, Salloway et al., 2014, Wisniewski and Goni, 2014). This disconnect emphasizes the need to introduce alternative and relevant functional end points during the preclinical evaluation of therapeutic candidates. We have previously reported that ∼20% of neurites (neuronal dendrites and axons) exhibit elevated levels of baseline calcium (calcium overload) that is correlated with disrupted neuronal structure and function in aged APP mice, a model that develops an amyloid pathology similar to that seen in AD (Kuchibhotla et al., 2008). Because increased calcium levels relate to neuronal activity, amelioration of this calcium overload could serve as an indirect functional indicator of treatment efficacy. To this end, we evaluated the effects of chaducanumab, a murine analog of the anti-Aβ antibody aducanumab, on calcium homeostasis and plaque clearance in Tg2576 mice (Dunstan et al., 2011).
Aducanumab is a human antibody that binds selectively to aggregated forms of Aβ, including insoluble fibrils and soluble oligomers. In a small phase 1b study in prodromal or mild AD subjects, aducanumab was shown to reduce amyloid deposition in the brain in a dose- and time-dependent manner and to slow cognitive decline in a dose-dependent manner (Sevigny et al., 2016). chaducanumab, the murine chimeric analog of aducanumab, was used in the present rodent study because it accurately mimics immune effector function in mice. chaducanumab was applied directly onto the brain of living 22-month-old transgenic Tg2576 mice. Multiphoton microscopy (Kastanenka et al., 2015) showed clearance of existing amyloid plaques 3 weeks after chaducanumab treatment. Even though a 6-month-long systemic treatment with chaducanumab did not lead to amyloid plaque clearance, calcium overload in neurons in Tg2576 mice was ameliorated. This suggests that chaducanumab may be targeting soluble species of Aβ and slowing cognitive decline by restoring calcium homeostasis and proper neuronal function.
Materials and Methods
Animals and surgery.
Transgenic Tg2576 mice (APPSwe) at 18 or 22 months of age and nontransgenic control mice at 13 months of age were used (Taconic, RRID:IMSR_TAC:1349). Both sexes were included in the studies. The studies were conducted in accordance with Massachusetts General Hospital Animal Care and Use Committee and National Institutes of Health guidelines for the use of experimental animals.
In the acute immunotherapy condition, animals underwent a craniotomy and durotomy and received a topical application of the antibody as in previous experiments (Bacskai et al., 2001). IgG2a murine chimeric chaducanumab, or isotype matched P1.17 control antibody that does not recognize an epitope in mouse were applied to the brains. Then, animals underwent cranial window implantation over somatosensory cortex as described previously (Kuchibhotla et al., 2008). Briefly, mice were anesthetized with isoflurane and placed in a stereotaxic apparatus. Their body temperature was maintained with a heating pad throughout the course of anesthesia. The scalp was disinfected with betadine and isopropyl alcohol before incision. A round window was made over the brain by drilling and removing the skull. Each mouse received a topical application of the antibody (0.42–1 mg/ml) to the brain for 20 min. Subsequently, the site was covered with a glass coverslip 8 mm in diameter and fixed with a mixture of dental cement and crazy glue. One day before the craniotomy, animals received an intraperitoneal injection of methoxy-XO4 (10 mg/kg), which crossed the blood–brain barrier and labeled amyloid plaques (Klunk et al., 2002). Mice were 22 months old when they received the acute antibody treatment.
In the chronic immunotherapy condition, animals received intraperitoneal injections of the antibody (described below) after intracortical virus injections and cranial window installation as described previously (Kuchibhotla et al., 2008). Briefly, before AAV8-CBA-YC3.6 (University of Pennsylvania) injections, mice were anesthetized with isoflurane and secured in a stereotaxic apparatus. The scalp was disinfected over the injection site. Burr holes were drilled through the skull in each hemisphere with the following coordinates A–P 0, M–L ±1, D–V −1.5 with respect to bregma. Using a Hamilton syringe, 1–1.5 μl of virus (2 × 1012 molecules/ml) was injected 1.5 mm below the surface of dura at a rate of 0.13 μl/min to target neurons in the somatosensory cortex. After injections, a cranial window of 8 mm was installed over the brain areas injected with YC3.6 as described above. After the surgeries, mice were allowed to recover from anesthesia on a heating pad. The mice were prepared for imaging 3–4 weeks after injection of virus. Mice received an intraperitoneal injection of methoxy-XO4 (10 mg/kg) and imaged with multiphoton microscopy through cranial windows the following day.
Chronic immunotherapy treatment.
The mice in the chronic immunotherapy condition received intraperitoneal injections of chaducanumab (10 mg/kg) or control antibody P1.17 (10 mg/kg) weekly for 6 months starting immediately after the baseline imaging session. Mice were 18 months of age at the start of treatment. The first two injections were made using Cy3-labeled antibodies to determine whether antibodies crossed the blood–brain barrier and colocalized with methoxy-positive plaques. Mice were imaged at baseline, 2 weeks after treatment initiation, and monthly in the course of the chronic immunotherapy treatment.
Multiphoton imaging and data acquisition.
Imaging of YC3.6-filled neurons and amyloid plaques was performed using an Olympus Fluoview 1000MPE mounted on an Olympus BX61WI upright microscope (Kastanenka et al., 2015). A 25× water-immersion objective (numerical aperture 1.05) was used to visualize the brain and acquire images. A mode-locked titanium/sapphire laser (MaiTai; Spectra-Physics) generated two-photon fluorescence with 800 or 860 nm excitation. Detectors containing three photomultiplier tubes (Hamamatsu) collected light in the range of 380–480, 500–540, and 560–650 (Bacskai et al., 2001). Amyloid plaque pathology was imaged using 800 nm excitation at 1× magnification. A number or random z-stacks were taken within each cranial window at the first imaging session. The same regions within the cranial window were identified at later imaging sessions and reimaged. YC3.6-filled neurites and cell bodies were imaged with 860 nm excitation at 1×, 2×, and 5× magnification. Laser power was maintained <50 mW at the microscope back aperture to avoid phototoxicity. Image stacks were acquired at 1–5 μm steps.
At the end of each imaging session, mice were allowed to recover on a heating pad. For subsequent imaging sessions, mice were injected with methoxy-XO4 the day before imaging, anesthetized, and imaged using the multiphoton microscope as described above. After the last imaging session, mice were killed with CO2, perfused with 4% paraformaldehyde in PBS and postfixed in 4% paraformaldehyde with 15% glycerol cryoprotectant for 24 h. Brains were frozen in M1 mounting medium on ice-cold isopropanol, cut into 20 μm coronal sections, and mounted onto slides. Amyloid plaques labeled with methoxy-XO4 were quantified. Four to 11 images per brain were acquired on a Zeiss Observer Z1 microscope with a 5× objective using MetaMorph software.
Image processing and data analysis.
Image stacks acquired with multiphoton microscopy were analyzed using ImageJ. Images were processed and analyzed to elucidate amyloid plaque numbers and burden as well as baseline calcium. Because the same volumes of the brain before treatment and in the course of treatment were compared, each z-stack was verified to include the cells imaged in z-stacks acquired in prior imaging sessions for each brain volume. Cells not present in prior sessions were excluded from z-stacks analyzed. For amyloid plaque analysis, each z-stack was processed into a maximum intensity projection. Amyloid plaques were then manually counted in each projected image and compared before and after antibody treatment. For amyloid burden, each projected image was thresholded, segmented, and the percentage area occupied by amyloid was measured and represented as burden per cubic millimeter. Signal from amyloid lining the blood vessels was excluded from analyses. Amyloid plaque burden was calculated for cortical brain volumes imaged in vivo with multiphoton microscopy and cortical volumes and hippocampi imaged postmortem.
YC3.6 images were processed and analyzed using ImageJ. For both cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) channels, the background, corresponding to the mode at the last slice of each volume, was subtracted and a median filter with radius 2 was applied before dividing the emitted fluorescence intensity of YFP by CFP, thus creating a ratio image. Neurites and cell bodies were identified and selected using the YFP images either manually using the free hand tool or by adjusting the threshold and using the wand tool of the software. Selected regions of interest were then placed on the ratio images. Relative changes in the YFP/CFP ratio (ΔR/Ri) were evaluated by selecting the same neurites or soma before and after treatment. Mice in which cranial windows failed to remain clear and thus made finding and analyzing same neurites impossible were excluded from the study.
YFP/CFP ratios were converted to [Ca2+]i with standard equations using the in situ Kd and Hill coefficient of YC3.6 that we determined previously (Kuchibhotla et al., 2008). Pseudocolored images were created in MATLAB based on the YFP/CFP ratio, which was then converted to calcium concentration using the empirical Rmin and Rmax and assigned to the jet colormap. The ratio values were used to supply the hue and saturation (color) and the brightness values were used to supply the value (intensity).
Immunohistochemistry.
Mouse brains were processed as described above. Cross-sections underwent antigen retrieval treatment in citrate buffer and were incubated with antibodies against NR1 (mouse, 100 μg/ml, Millipore; RRID:AB_390129), NR2A (rabbit, 40 μg/ml Millipore; RRID:AB_310837), NR2B (rabbit, 20 μg/ml Millipore; RRID:AB_90772), Ryr (rabbit, 4 μg/ml Abcam; AB_1566701), SERCA2 (rabbit, 10 μg/ml Abcam), VILIP-1 (rabbit, 1.25 μg/ml, Abnova; RRID:AB_1581891), IP3 receptor (rabbit, 1.3 μg/ml, Abcam) for 2 h at room temperature followed by incubation with the appropriate secondary antibodies for 1 h and mounted with ProLong Antifade reagent (Invitrogen).
Statistics.
Statistical analyses were performed in GraphPad version 5.0. Data are expressed as mean ± SEM. Datasets were tested for normality (Shapiro–Wilk normality test, D'Agostino and Pearson omnibus normality test, or Kolmogorov–Smirnov test), after which the appropriate statistical tests were used (one-way t test or ANOVA for normally distributed data, Mann–Whitney or Kruskal–Wallis test for nonparametric data). For datasets comparing 2 conditions, p < 0.05 was considered significant. For datasets comparing 3 conditions, p < 0.025 was considered significant.
Results
Topical application of chaducanumab clears plaques
Amyloid plaque burden and amyloid plaque numbers were assessed after topical application of chaducanumab directly to the surface of the brain. chaducanumab, a chimeric analog containing the intact variable domains of aducanumab, murine IgG2a heavy-chain, and murine κ light-chain constant domains, was used instead of aducanumab itself because it mimics accurately the immune effector function in mice (e.g., the ability to bind Fcγ receptors and recruit phagocytic cells). chaducanumab also reduces the likelihood of an antidrug response after repeat dosing in mice. chaducanumab (0.4–1 mg/ml) was applied directly onto the brain of living 22-month-old transgenic Tg2576 mice after craniotomy and durotomy. Multiphoton microscopy was used to image methoxy-XO4 labeled plaques on the day of the topical application and 3 weeks later to determine the effect of chaducanumab on clearance of existing amyloid plaques and deposition of new plaques (Fig. 1A). We used landmarks in the mouse brains, such as cerebral amyloid angiopathy (CAA), to find the same brain regions and identify individual plaques reliably. Tg2576 mice used in this study had substantial CAA, the pattern of which was not altered with the antibody treatment. Acute topical application of chaducanumab led to plaque clearance, determined as a decrease in the amyloid plaque number (Fig. 1B–E,L). A total of 48 ± 4% of plaques disappeared after chaducanumab treatment compared with only 14 ± 3% of plaques disappearing after treatment with a control antibody, P1.17 (Fig. 1J, Mann–Whitney test, p < 0.0001, n = 31 z-stacks in 3 mice treated with chaducanumab, n = 39 z-stacks in 4 mice treated with the control antibody). Furthermore, a decrease in size of 13 ± 2% of the remaining individual plaques was observed after chaducanumab treatment (Fig. 1M). Acute application of chaducanumab did not prevent deposition of new plaques significantly (Fig. 1B,D,K; chaducanumab: 34 ± 5% of new plaques appeared vs control: 44 ± 9%; Mann–Whitney test, p = 0.98; n = 31 z-stacks in 3 mice treated with chaducanumab, n = 39 z-stacks in 4 mice treated with the control antibody). Overall amyloid plaque burden was reduced within the imaged volumes (Fig. 1B–E,O). Treatment with an isotype-matched monoclonal antibody with no known epitope in mouse brain, P1.17, did not result in amyloid plaque clearance (Fig. 1F–J), and there was a modest increase in amyloid burden in these mice over the 3 week period (Fig. 1O). Aducanumab treatment resulted in 27 ± 8% reduction in amyloid plaque burden, whereas treatment with the control antibody resulted in a 33 ± 15% increase in amyloid burden (Fig. 1N; Mann–Whitney test, p < 0.0001; n = 31 z-stacks in 3 mice treated with aducanumab, n = 39 z-stacks in 4 mice treated with the control antibody). Therefore, a single topical application of chaducanumab led to a rapid decrease in amyloid burden as measured with longitudinal imaging.
Chronic treatment with chaducanumab does not affect existing plaques in very old Tg2576 mice
Brain penetration of the antibody was first determined using Cy3-tagged chaducanumab injected intraperitoneally and colocalization with methoxy-XO4-positive plaques indicated target engagement in the brain (Fig. 2A,B). The control antibody also crossed the blood–brain barrier because Cy3 signal was visualized in mouse brains; however, it failed to colocalize with amyloid plaques (Fig. 2C,D). Six months of chronic treatment was initiated in 18-month-old animals that were imaged before antibody treatment (baseline), 2 weeks after onset of treatment, and once a month thereafter during the course of the treatment (Fig. 3A). Intraperitoneal injections of methoxy-XO4 were made before each imaging session. Because the same brain areas were imaged longitudinally in each mouse, amyloid plaque burden was determined and could be compared across imaging sessions. Amyloid plaque numbers were counted for the first and last imaging sessions. There was no substantial plaque clearance after the 6 month treatment period in either the chaducanumab- or the control-treated animals (chaducanumab: 4 ± 1% of amyloid plaques were cleared; control: 5 ± 2% of plaques cleared; Mann–Whitney test, p = 0.35; n = 42 z-stacks in 5 mice treated with chaducanumab, n = 22 z-stacks in 3 mice treated with the control antibody), nor was there any evidence of substantial new plaque appearance (chaducanumab: 8.5 ± 2% of new plaques appeared, control: 6 ± 3% of new plaques appeared; Mann–Whitney test, p = 0.06; n = 42 z-stacks in 5 mice treated with chaducanumab, n = 22 z-stacks in 3 mice treated with the control antibody) after control antibody (Fig. 3E–K) or chaducanumab (Fig. 3L–R) treatment. Therefore, the net change in plaque number (Fig. 3B) or amyloid plaque burden (Fig. 3C) was not significant for either condition. The size of plaques was not altered as a result of chaducanumab or control antibody treatment over the 6 month period (46 ± 4% of amyloid plaques increased in size after chaducanumab treatment vs 35 ± 6% of plaques increased in size after control antibody treatment; Mann–Whitney test, p = 0.12; n = 42 z-stacks in 5 mice treated with chaducanumab, n = 22 z-stacks in 3 mice treated with the control antibody). Because in vivo multiphoton microscopy allows imaging of amyloid plaques in a small cortical volume, we confirmed the effects of immunotherapy using postmortem evaluation of amyloid burden in the cortex and hippocampus. After the final imaging session, brains were isolated, processed, and sectioned to allow for ex vivo quantification of methoxy-XO4-labeled amyloid plaques. Cortical amyloid plaque burden from in vivo imaging on the last imaging session was comparable to total cortical amyloid plaque burden analyzed postmortem and similar to that in hippocampus (Fig. 3D).
Aged Tg2576 mice exhibit calcium overload
Calcium homeostasis plays a major role in maintaining neuronal function. Resting calcium levels are elevated in aged APP mice, resulting in calcium overload (Kuchibhotla et al., 2008). We tested whether chronic treatment with chaducanumab could restore neuronal calcium to control levels. Therefore, calcium dynamics were used as a measure of functional outcome.
First, we verified the presence of calcium overload in 18-month-old Tg2576 mice. Calcium was monitored using the ratiometric probe yellow cameleon 3.6 (YC3.6), which was virally expressed in the somatosensory cortex using an adeno-associated viral (AAV) vector before imaging (Fig. 3A). YC3.6 contains a donor, CFP, and an acceptor, YFP (Nagai et al., 2004). Binding of calcium to the probe leads to increased FRET efficiency and elevation in the ratio of YFP to CFP. Intracellular calcium concentrations can be derived directly from the ratio of YFP to CFP. Calcium overload was defined as a level >2 SDs above the mean of control cells. A ratio of 1.74 constituted calcium overload and translated into 200 nm intracellular calcium concentration. Using multiphoton microscopy through cranial windows, we measured intracellular calcium concentration within neurites in 18-month-old transgenic Tg2576 mice when a substantial amyloid burden was present and in wild-type mice. A total of 18.5% of transgenic mouse neurites exhibited calcium overload, whereas only 4.0% of wild-type mouse neurites had elevated levels of calcium (Fig. 4A,B, black arrows) (Kuchibhotla et al., 2008). Therefore, aged Tg2576 mice exhibit elevated levels of baseline calcium within neurites.
Chronic treatment with chaducanumab restores intracellular calcium to control levels in aged Tg2576 mice
The effect of chronic treatment with chaducanumab on neuronal calcium overload was assessed in Tg2576 mice, which received systemic injections of chaducanumab or control antibody at 10 mg/kg weekly for 6 months starting at 18 months of age. YC3.6 was visualized before antibody treatment, 2 weeks after onset of treatment, and monthly thereafter in cortical neurites over the 6 month treatment period with multiphoton microscopy (Fig. 3A). Care was taken to image the same brain volumes during each imaging session and to select the same identified neurites during data analysis to make reliable comparisons with baseline calcium levels within each individual neurite over time. We used landmarks in the mouse brains such as neurite patterns, CAA, and amyloid plaques, the presence and location of which were comparable for a given brain across imaging sessions (Fig. 3E–R). Systemic administration of chaducanumab restored neurite baseline calcium to control levels (Fig. 4C,D, yellow arrowheads). A total of 18.5% of neurites exhibited calcium overload at the start of treatment at 18 months (Fig. 4A, black arrow, H). Remarkably, chaducanumab decreased the number of neurites with elevated levels of calcium 2 weeks after the start of treatment (18.5 months vs 18 months in Fig. 4A,H). By the end of 6 months of treatment, at 24 months of age, mice treated with chaducanumab had no neurites with elevated levels of calcium (Fig. 4A,H), whereas mice treated with the control antibody maintained elevated (28%) calcium levels in neurites (Fig. 4E,F, green arrowheads, G,H). Because individual neurites were identified and followed longitudinally, this reversal of calcium overload is not the result of neurite loss, but rather represents a true restoration of normal resting calcium. Furthermore, treatment with chaducanumab did not alter baseline calcium in wild-type mice (Fig. 4B), suggesting that chaducanumab ameliorated calcium overload by interacting with human Aβ that Tg2576 mice produce due to overexpression of human APP and not by interacting with endogenous mouse Aβ. Therefore, chaducanumab restored resting calcium to control levels and prevented neurite calcium overload in all neurites imaged (chaducanumab: 100% of neurites that started out with calcium overload had their calcium restored to control levels vs 4 ± 3% of neurites in control antibody condition; Mann–Whitney test, p < 0.05; n = 139 neurites in 5 mice treated with chaducanumab, n = 135 neurites in 3 mice in control antibody condition).
Furthermore, calcium levels were assessed in neuronal cell bodies. At the onset of treatment, Tg2576 mice exhibited limited calcium overload within their neuronal cell bodies (5% in control antibody treatment condition, 0% in the chaducanumab treatment condition). The percentage of cell bodies exhibiting calcium overload increased in the course of treatment with the control antibody (Fig. 4I,K). Interestingly, chaducanumab treatment prevented this increase (Fig. 4J,K).
NMDA receptors are downregulated in Tg2576 mice exhibiting calcium overload
To gain insight into the cellular processes associated with neuronal calcium overload, we performed immunohistochemistry for a variety of calcium-homeostasis-related proteins in the mouse brains at the end of the chronic treatment period. The levels of the calcium sensor visinin-like protein (VILIP) (Tarawneh et al., 2011, Lu et al., 2015) were increased within neurites and cell bodies in Tg2576 mouse brains compared with the wild-type mice (Fig. 5A,B,D,E). Treatment with chaducanumab restored the levels of VILIP to control levels (Fig. 5A,C,D,E), corresponding to the restoration of calcium overload (Fig. 4A,H).
The NMDA receptor subunit NR1 and the NR2 subunits permeable to calcium, specifically NR2A and NR2B, were also assessed. The overall levels of these proteins were similar in the wild-type mice and the transgenic mice treated with control or chaducanumab antibodies (Fig. 5F–H,K–M,P–R,I,N,S). However, when the numbers of cells within cortex were counted in the tissue sections positive for NR1, there were fewer NR1-positive neurons in Tg2576 mice treated with the control antibody compared with the wild-type controls (Fig. 5F–H,J). The number of NR2A- and NR2B-expressing cells also decreased in the mice treated with control antibody compared with wild-type mice (Fig. 5K–M,O,P–R,T). Treatment with chaducanumab restored the cell numbers to normal for NR1 and NR2A, but not NR2B (Fig. 5J,O,T).
Finally, the levels of proteins involved in regulation of internal calcium stores including calcium ATPase SERCA, IP3 receptor, and ryanodine receptor (RyR) (Rizzuto, 2001) were assessed. The number of SERCA positive cells did not differ significantly between conditions (Fig. 6A–C,E). However, levels of SERCA within the cells were reduced in Tg2576 mice treated with control antibody and restored to normal levels with chaducanumab treatment (Fig. 6A–D). Neither the cell number nor the levels of IP3 receptor and RyR was different between the two treatment groups or in wild-type mice (Fig. 6F–O). These data suggest that the calcium overload is likely associated with aberrant entry of calcium from extracellular sources and that calcium homeostasis is restored by chaducanumab treatment.
Discussion
We evaluated the effects of aducanumab, an anti-Aβ antibody currently being evaluated in clinical trials for the treatment of AD, on amyloid plaque clearance and restoration of intraneuronal calcium levels. Aducanumab is a human antibody that binds selectively to aggregated forms of Aβ, including insoluble fibrils and soluble oligomers. We demonstrated that acute topical application of chaducanumab, the murine analog of aducanumab, to the brain of Tg2576 mice resulted in clearance of existing amyloid plaques. Brain penetration and target engagement were demonstrated after systemic injection of the labeled antibody. Multiphoton microscopy was used for longitudinal imaging of very old Tg2576 mice treated with the antibody. Chronic systemic administration over 6 months with chaducanumab failed to clear existing amyloid plaques in these mice (18–24 months of age), consistent with the notion that immunotherapy is more effective for the prevention or treatment of amyloidosis at earlier stages and not as effective in advanced stages with substantial parenchymal plaque deposits. In addition, plaques in older animals might be resistant to anti-amyloid therapy, as was indicated in an earlier study (Demattos et al., 2012). However, despite the lack of detectable plaque clearance, a robust effect on intraneuronal calcium levels was observed. Future studies will be necessary to elucidate the effect of chaducanumab on calcium dynamics in astrocytes. Neuronal calcium dysregulation may be a mediator of progressive neurotoxicity (Kuchibhotla et al., 2008) and sustained elevations lead to activation of downstream effectors including the calcium-sensitive phosphatase calcineurin, which is associated with neurite pruning, loss of synapses, and neurotoxicity (Wu et al., 2010). Although no overt neurite pruning or cell body loss was observed in this study, a rigorous assessment will be necessary in future studies. Altered calcium homeostasis also leads to a shift in the balance of synaptic plasticity from an LTP-like state to one more resembling LTD (Berridge, 2011). Altogether, the dysregulation of intracellular calcium levels is likely to lead to functional abnormalities in neuronal networks that possibly underlie memory deficits in humans. Therefore, it is notable that anti-amyloid immunotherapy with chaducanumab in Tg2576-transgenic mice can reverse this pathophysiological phenomenon and suggests that cognitive improvements can occur in the absence of overt amyloid plaque clearance.
A plausible explanation for this apparent discrepancy could be related to the specificity of chaducanumab for aggregated forms of Aβ. It has been demonstrated that Aβ oligomers are mediators of neurotoxicity and are likely to be the Aβ species that initiates the cascade of neurodegeneration (Crimins et al., 2013, Lesné et al., 2013). Therefore, the ability to target this species, in addition to clearing amyloid plaques, has important implications for the development of therapeutics. chaducanumab recognizes amyloid aggregates that includes soluble oligomers, but not monomers, of Aβ. Therefore, this conformation-dependent antibody is well poised to not only avoid the overwhelming concentration of monomeric Aβ in both plasma and brain parenchyma, but also to target the most relevant neurotoxic species in the brain. One can hypothesize that, by capturing soluble Aβ oligomers, chaducanumab would prevent their binding to neuronal receptors known to be involved in calcium regulation, therefore preventing the calcium overload previously described in the brain of APP-transgenic mice.
To address the mechanisms of calcium dyshomeostasis and reversal with chaducanumab, we used immunohistochemical analysis of calcium related proteins in the brain. There were elevated levels of VILIP in cortices of Tg2576 mice treated with control antibody compared with levels in cortices of mice treated with chaducanumab. VILIP-1 has been identified as a brain injury biomarker and its levels are upregulated in AD patients (Tarawneh et al., 2011, Lu et al., 2015). Furthermore, whereas the NMDA receptor subunits NR1, NR2A, and NR2B were downregulated in APP mice, treatment with chaducanumab restored the levels of NR1 and NR2A, but not NR2B. It has been suggested that, in addition to being important as a developmental switch, the ratio of NR2A:NR2B also increases the susceptibility to excitotoxicity through the NMDA receptor (Tang et al., 1999, Cui et al., 2013). The expression of the intracellular store channel SERCA was reduced in Tg2576 mice treated with control antibody and restored with chaducanumab immunotherapy, suggesting that intracellular calcium stores may contribute to calcium dyshomeostasis. However, RyR, calcium ATPase, and IP3 receptor levels were not different in transgenic compared with wild-type mice, suggesting that intracellular stores likely play a minor role.
In conclusion, treatment with chaducanumab led to the improvement of a functional outcome measure without reducing the amyloid load significantly after chronic treatment in very old mice. Therefore, we believe that an assay reflective of neuronal physiology, such as calcium imaging, might be considered as an indirect functional outcome measure. Assessment of cognitive deficits with behavioral tests in mice is conceptually appealing because this represents a direct functional end point. However, studies with anti-Aβ immunotherapy have led to conflicting results, with effects on parenchymal Aβ often accompanied by no or very minor reversal of memory deficits (Arendash et al., 2001, Austin et al., 2003, Adolfsson et al., 2012, Bohrmann et al., 2012). Because chaducanumab restored calcium overload, this antibody may have beneficial effects on neuronal network function that likely underlie cognitive deficits and is thus a promising treatment for AD. The development of conformationally specific immunotherapy approaches offers the opportunity to target the relevant amyloid species that initiate the neurodegenerative cascade without the confounding factor of engaging irrelevant Aβ species that limits overall efficacy.
Footnotes
This work was supported by Biogen, the Alzheimer's Association, and the National Institutes of Health (Grants R01EB000768, R01AG044263, P30NS045776, and S10RR025645).
This research was funded by Biogen. The authors declare no competing financial interests.
- Correspondence should be addressed to either Brian J. Bacskai or Ksenia V. Kastanenka, Department of Neurology, MassGeneral Institute of Neurodegenerative Diseases, Massachusetts General Hospital and Harvard Medical School, 114 Sixteenth St., Charlestown, MA 02129. bbacskai{at}mgh.harvard.edu or kkastanenka{at}mgh.harvard.edu