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Journal of Neuroscience, Vol 1, 505-513, Copyright © 1981 by Society for Neuroscience
Two components from eye tissue that differentially stimulate the growth and development of ciliary ganglion neurons in cell culture
R Nishi and DK Berg
Survival and development of chick ciliary ganglion neurons in vivo appear
to depend on information from the embryonic eye structure that contains the
postsynaptic targets of the neurons. We have tested embryonic eye extracts
on ciliary ganglion neurons in dissociated cell culture for stimulation of
growth and development. Control conditions were chosen that permitted the
long term maintenance of the neurons in the absence of tissue extracts of
conditioned medium. The conditions included coating the culture substratum
with fibroblast material and increasing the K+ concentration in the culture
medium to 25 mM. Neurons survived for at least 3 weeks in control
conditions. Two major components were resolved in eye extracts that
stimulated growth and development of the neurons above the basal levels
obtained with control conditions. One component, with an apparent molecular
weight of about 2 X 10(4) by gel filtration analysis, stimulated neuronal
growth without increasing the levels of choline acetyltransferase activity
per neuron. The second component, with an apparent molecular weight of
about 5 X 10(4), increased development of choline acetyltransferase levels
per neuron but had no effect on neuronal growth. Both components were
effective in normal K+ as well as 25 mM K+. These components may represent
mechanisms by which the postsynaptic target tissue acts in vivo to direct
the growth and development of ciliary ganglion neurons.
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