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Journal of Neuroscience, Vol 1, 703-709, Copyright © 1981 by Society for Neuroscience
Ontogenesis of olivocerebellar relationships. II. Spontaneous activity of inferior olivary neurons and climbing fibermediated activity of cerebellar Purkinje cells in developing rats
J Mariani and JP Changeux
During development of vertebrate cerebellum, a multiple innervation of each
Purkinje cell (PC) by several climbing fibers (CFs) (Crepel, F., J.
Mariani, and N. Delhaye-Bouchaud (1976) J. Neurobiol. 7: 567-578; Mariani,
J., and J. -P. Changeux (1981) J. Neurosci. 1: 696-702) precedes the
one-to-one relationship characteristic of the adult stage. In the present
study, the spontaneous activity of inferior olivary neurons (whose axons
constitute the CFs) and the spontaneous activity elicited in PCs by the CF
system were investigated in developing rats. Extracellular unitary
recordings were obtained in medial and dorsal accessory olives from 249
neurons from 1-day-old to adult animals. The mean discharge rate varied
from 0.19 spike . sec-1 on day 1 to 1.69 spikes . sec-1 on postnatal day 21
and 1.78 spikes . sec-1 in adult animals. Although the spontaneous
discharge was low, interspike interval histograms were constructed for a
sample of cells. Their modal value varied from a mean value of 660 msec on
day 1 to 250 msec in the adult with, however, marked variations at each age
in the young animals. Intracellular recordings from PCs were performed in
rats ranging in age from 5 to 15 postnatal days. The spontaneous (i.e.,
asynchronous) discharge of excitatory postsynaptic potentials mediated
neurons at the same age. The main results are the following: (1) In PCs
innervated by one CF, the mean frequency of CF-EPSPs was similar to the
discharge of inferior olivary neurons; (2) in PCs innervated by several
CFs, the mean discharge of CF-EPSPs was much higher than the discharge of
inferior olivary neurons; (3) as a whole, the CF-mediated activity of PCs
decreased from day 5 to day 11, while the multiple innervation disappeared;
from then on, the activity increased with the maturation of inferior
olivary neurons until adult values. The ratio of the frequencies of
CF-EPSPs and olivary neurons discharges gives an approximate mean number of
CF collaterals per PC at each age; it varied from 3.6 on day 5 to values
close to 1 on day 11 and the following days.
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