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Journal of Neuroscience, Vol 1, 1008-1021, Copyright © 1981 by Society for Neuroscience
Neurite outgrowth in molluscan organ and cell cultures: the role of conditioning factor(s)
RG Wong, RD Hadley, SB Kater and GC Hauser
Isolated neurons from adult central ganglia of the snail Helisoma were
cultured in vitro in modified Liebowitz L-15 medium. Such neurons displayed
electrical excitability comparable to that in acutely dissected ganglia.
Isolated neurons remained spherical in defined medium throughout culture
durations up to 2 weeks. This static morphology was contrasted by the
significant neuritic outgrowth which occurred from neurons maintained in
medium with co-cultured intact Helisoma brains or in brain conditioned
medium. A morphological sequence of growth cone formation and neurite
extension occurred only in the presence of a conditioning factor(s) with a
mode of action which included tight binding of the conditioning factor to
the substratum. Under these conditions, the two primary neuronal
phenotypes, electrical excitability and complex neuronal architecture,
could be affected independently in adult molluscan neurons cultured in
vitro.
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