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Journal of Neuroscience, Vol 10, 317-322, Copyright © 1990 by Society for Neuroscience


ARTICLE

Multiple GTP-binding proteins from cholinergic synaptic vesicles

JK Ngsee, K Miller, B Wendland and RH Scheller
Department of Biological Sciences, Stanford University, California 94305.

Cholinergic synaptic vesicles purified from the electric organ of the marine ray, Discopyge ommata, contain 2 different size classes of GTP- binding proteins: one or more with an apparent molecular weight (MW) between 37 and 41 kDa, and 3 major and at least 2 minor proteins with MW between 20 and 29 kDa. These GTP-binding proteins were detectable using the alpha 32P-GTP overlay technique and covalent modification with bacterial toxins. The higher MW GTP-binding proteins are ADP- ribosylated by pertussis toxin and 2 of the lower MW GTP-binding proteins are sensitive to botulinum toxin.


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