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Journal of Neuroscience, Vol 11, 2972-2984, Copyright © 1991 by Society for Neuroscience
Molecular cloning and sequencing of general odorant-binding proteins GOBP1 and GOBP2 from the tobacco hawk moth Manduca sexta: comparisons with other insect OBPs and their signal peptides
RG Vogt, R Rybczynski and MR Lerner
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
Odorant-binding proteins (OBPs) are small, water-soluble proteins uniquely
expressed in olfactory tissue of insects and vertebrates. OBPs are present
in the aqueous fluid surrounding olfactory sensory dendrites and are
thought to aid in the capture and transport of hydrophobic odorants into
and through this fluid. OBPs may represent the initial biochemical
recognition step in olfaction, because they transport odorants to the
receptor neurons. Insect OBPs are represented by multiple classes:
pheromone-binding proteins (PBPs) and general odorant-binding proteins
(GOBP1 and GOBP2). PBPs associate with pheromone-sensitive neurons, while
GOBPs associate with general odorant- sensitive neurons. Analysis of
N-terminal amino acid sequences of 14 insect OBPs isolated from six species
indicated that the PBPs were variable and the GOBPs were highly conserved.
However, inferred properties of these proteins were based only on partial
sequence data. We now report the full-length sequences of a GOBP1 and GOBP2
from the moth Manduca sexta and compare these sequences with those of PBPs
from three species, including M. sexta, Antheraea polyphemus, and A.
pernyi. We also compare these with a GOBP2 of A. pernyi, previously
identified only as a novel OBP. These comparisons fully support our
N-terminal analysis. The signal peptide sequences of seven insect OBPs
reveal conserved sequences within OBP classes, but not between OBP classes
even within the same animal species. This suggests that multiple OBPs may
be coexpressed in the same cell type, but differentially processed in a
class-specific manner. Properties of the GOBPs suggest that general
olfaction is broadly receptive at the periphery. Properties of the PBPs
suggest that pheromone olfaction is discriminatory at the periphery, and
that the initial biochemical steps in pheromone detection may play an
active role in odor perception.
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