QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (214) Citing Articles via Google Scholar Google Scholar Articles by Santos-Sacchi, J. Search for Related Content PubMed PubMed Citation Articles by Santos-Sacchi, J.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 11, 3096-3110, Copyright © 1991 by Society for Neuroscience

ARTICLE

Reversible inhibition of voltage-dependent outer hair cell motility and capacitance

J Santos-Sacchi
Laboratory of Otolaryngology, UMDNJ-New Jersey Medical School, Newark 07103.

Outer hair cells (OHC) from the organ of Corti are capable of fast voltage-induced length changes (Santos-Sacchi and Dilger, 1988), suggesting that an associated voltage sensor should reside in the OHC plasma membrane. Voltage-dependent mechanical responses and nonlinear charge movement of isolated OHCs from the guinea pig were analyzed using the whole-cell voltage-clamp technique. Ionic currents in the cells were blocked. Nonlinear voltage-dependent charge movement or, correspondingly, voltage-dependent capacitance was measured with step or AC analysis. OHC movements were measured either from video or using a differential photodiode technique. Maximum charge movements up to 2.5 pC were measured in OHCs from the low-frequency region of the cochlea. Both AC and step analyses indicated a peak nonlinear capacitance of 16- 17 pF. The voltage dependence was fit to a Boltzmann relation with the step analysis indicating a maximum nonlinear capacitance at -23 mV step potential from a holding potential of about -120 mV, and AC analysis indicating a maximum at a holding potential near -40 mV. AC analysis probably provides a more accurate evaluation of voltage dependence. Measures of OHC motility magnitude versus voltage follow the nonlinear capacitance-voltage function obtained from AC measures. Treatment of the cells with gadolinium ions (0.5-1 mM) blocked OHC motility. This treatment also produced a shift of the nonlinear capacitance function along the voltage axis in the depolarizing direction, which can be explained by membrane surface charge screening. However, maximum capacitance was reduced as well and may correspond to the reduction or abolition of OHC motility in response to gadolinium treatment. Gadolinium effects were reversible. Nonlinear capacitance is not a function of membrane deformation due to length changes, since removal of OHC cytosol via the patch pipette abolished longitudinal movement but did not reduce nonlinear charge movement. It is interesting to note that the nonlinear capacitance will dynamically influence the time constant of the OHC during acoustically evoked receptor potential generation.

This article has been cited by other articles:

				K. R. Gleitsman, M. Tateyama, and Y. Kubo Structural rearrangements of the motor protein prestin revealed by fluorescence resonance energy transfer Am J Physiol Cell Physiol, August 1, 2009; 297(2): C290 - C298. [Abstract] [Full Text] [PDF]

				J. Sfondouris, L. Rajagopalan, F. A. Pereira, and W. E. Brownell Membrane Composition Modulates Prestin-associated Charge Movement J. Biol. Chem., August 15, 2008; 283(33): 22473 - 22481. [Abstract] [Full Text] [PDF]

				S. Detro-Dassen, M. Schanzler, H. Lauks, I. Martin, S. M. z. Berstenhorst, D. Nothmann, D. Torres-Salazar, P. Hidalgo, G. Schmalzing, and C. Fahlke Conserved Dimeric Subunit Stoichiometry of SLC26 Multifunctional Anion Exchangers J. Biol. Chem., February 15, 2008; 283(7): 4177 - 4188. [Abstract] [Full Text] [PDF]

				J. Ashmore Cochlear Outer Hair Cell Motility Physiol Rev, January 1, 2008; 88(1): 173 - 210. [Abstract] [Full Text] [PDF]

				L. Rajagopalan, J. N. Greeson, A. Xia, H. Liu, A. Sturm, R. M. Raphael, A. L. Davidson, J. S. Oghalai, F. A. Pereira, and W. E. Brownell Tuning of the Outer Hair Cell Motor by Membrane Cholesterol J. Biol. Chem., December 14, 2007; 282(50): 36659 - 36670. [Abstract] [Full Text] [PDF]

				A. Neef, D. Khimich, P. Pirih, D. Riedel, F. Wolf, and T. Moser Probing the Mechanism of Exocytosis at the Hair Cell Ribbon Synapse J. Neurosci., November 21, 2007; 27(47): 12933 - 12944. [Abstract] [Full Text] [PDF]

				M. Knirsch, N. Brandt, C. Braig, S. Kuhn, B. Hirt, S. Munkner, M. Knipper, and J. Engel Persistence of Cav1.3 Ca2+ Channels in Mature Outer Hair Cells Supports Outer Hair Cell Afferent Signaling J. Neurosci., June 13, 2007; 27(24): 6442 - 6451. [Abstract] [Full Text] [PDF]

				J. T. Albert, H. Winter, T. J. Schaechinger, T. Weber, X. Wang, D. Z. Z. He, O. Hendrich, H.-S. Geisler, U. Zimmermann, K. Oelmann, et al. Voltage-sensitive prestin orthologue expressed in zebrafish hair cells J. Physiol., April 15, 2007; 580(2): 451 - 461. [Abstract] [Full Text] [PDF]

				L. Rajagopalan, N. Patel, S. Madabushi, J. A. Goddard, V. Anjan, F. Lin, C. Shope, B. Farrell, O. Lichtarge, A. L. Davidson, et al. Essential Helix Interactions in the Anion Transporter Domain of Prestin Revealed by Evolutionary Trace Analysis J. Neurosci., December 6, 2006; 26(49): 12727 - 12734. [Abstract] [Full Text] [PDF]

				H. E. Farris, G. B. Wells, and A. J. Ricci Steady-State Adaptation of Mechanotransduction Modulates the Resting Potential of Auditory Hair Cells, Providing an Assay for Endolymph [Ca2+] J. Neurosci., November 29, 2006; 26(48): 12526 - 12536. [Abstract] [Full Text] [PDF]

				P. Dallos, J. Zheng, and M. A. Cheatham Prestin and the cochlear amplifier J. Physiol., October 1, 2006; 576(1): 37 - 42. [Abstract] [Full Text] [PDF]

				G. I. Frolenkov Regulation of electromotility in the cochlear outer hair cell J. Physiol., October 1, 2006; 576(1): 43 - 48. [Abstract] [Full Text] [PDF]

				J. Santos-Sacchi, L. Song, J. Zheng, and A. L. Nuttall Control of Mammalian Cochlear Amplification by Chloride Anions J. Neurosci., April 12, 2006; 26(15): 3992 - 3998. [Abstract] [Full Text] [PDF]

				H. J. Kennedy, M. G. Evans, A. C. Crawford, and R. Fettiplace Depolarization of cochlear outer hair cells evokes active hair bundle motion by two mechanisms. J. Neurosci., March 8, 2006; 26(10): 2757 - 2766. [Abstract] [Full Text] [PDF]

				M. A. Cheatham, J. Zheng, K. H. Huynh, G. G. Du, J. Gao, J. Zuo, E. Navarrete, and P. Dallos Cochlear function in mice with only one copy of the prestin gene J. Physiol., November 15, 2005; 569(1): 229 - 241. [Abstract] [Full Text] [PDF]

				J. Zheng, G.-G. Du, K. Matsuda, A. Orem, S. Aguinaga, L. Deak, E. Navarrete, L. D. Madison, and P. Dallos The C-terminus of prestin influences nonlinear capacitance and plasma membrane targeting J. Cell Sci., July 1, 2005; 118(13): 2987 - 2996. [Abstract] [Full Text] [PDF]

				L. Deak, J. Zheng, A. Orem, G.-G. Du, S. Aguinaga, K. Matsuda, and P. Dallos Effects of cyclic nucleotides on the function of prestin J. Physiol., March 1, 2005; 563(2): 483 - 496. [Abstract] [Full Text] [PDF]

				M. P. Scherer and A. W. Gummer Vibration pattern of the organ of Corti up to 50 kHz: Evidence for resonant electromechanical force PNAS, December 21, 2004; 101(51): 17652 - 17657. [Abstract] [Full Text] [PDF]

				M. Zhang, G. M. Kalinec, R. Urrutia, D. D. Billadeau, and F. Kalinec ROCK-dependent and ROCK-independent Control of Cochlear Outer Hair Cell Electromotility J. Biol. Chem., September 12, 2003; 278(37): 35644 - 35650. [Abstract] [Full Text] [PDF]

				V. Rybalchenko and J. Santos-Sacchi Cl- flux through a non-selective, stretch-sensitive conductance influences the outer hair cell motor of the guinea-pig J. Physiol., March 15, 2003; 547(3): 873 - 891. [Abstract] [Full Text] [PDF]

				D. Z Z He, K. W Beisel, L. Chen, D.-L. Ding, S. Jia, B. Fritzsch, and R. Salvi Chick hair cells do not exhibit voltage-dependent somatic motility J. Physiol., January 15, 2003; 546(2): 511 - 520. [Abstract] [Full Text] [PDF]

				A. Rusch, L. Ng, R. Goodyear, D. Oliver, I. Lisoukov, B. Vennstrom, G. Richardson, M. W. Kelley, and D. Forrest Retardation of Cochlear Maturation and Impaired Hair Cell Function Caused by Deletion of All Known Thyroid Hormone Receptors J. Neurosci., December 15, 2001; 21(24): 9792 - 9800. [Abstract] [Full Text] [PDF]

				D. Oliver, D. Z. Z. He, N. Klocker, J. Ludwig, U. Schulte, S. Waldegger, J. P. Ruppersberg, P. Dallos, and B. Fakler Intracellular Anions as the Voltage Sensor of Prestin, the Outer Hair Cell Motor Protein Science, June 22, 2001; 292(5525): 2340 - 2343. [Abstract] [Full Text] [PDF]

				J. Ludwig, D. Oliver, G. Frank, N. Klocker, A. W. Gummer, and B. Fakler Reciprocal electromechanical properties of rat prestin: The motor molecule from rat outer hair cells PNAS, March 27, 2001; 98(7): 4178 - 4183. [Abstract] [Full Text] [PDF]

				J. Santos-Sacchi, W. Shen, J. Zheng, and P. Dallos Effects of membrane potential and tension on prestin, the outer hair cell lateral membrane motor protin J. Physiol., March 15, 2001; 531(3): 661 - 666. [Abstract] [Full Text] [PDF]

				G. I Frolenkov, F. Mammano, and B. Kachar Action of 2,3-butanedione monoxime on capacitance and electromotility of guinea-pig cochlear outer hair cells J. Physiol., March 15, 2001; 531(3): 667 - 676. [Abstract] [Full Text] [PDF]

				I. A. Belyantseva, G. I. Frolenkov, J. B. Wade, F. Mammano, and B. Kachar Water Permeability of Cochlear Outer Hair Cells: Characterization and Relationship to Electromotility J. Neurosci., December 15, 2000; 20(24): 8996 - 9003. [Abstract] [Full Text] [PDF]

				J. F. Ashmore, G. S. G. Geleoc, and L. Harbott Molecular mechanisms of sound amplification in the mammalian cochlea PNAS, October 24, 2000; 97(22): 11759 - 11764. [Abstract] [Full Text] [PDF]

				G. I. Frolenkov, F. Mammano, I. A. Belyantseva, D. Coling, and B. Kachar Two Distinct Ca2+-Dependent Signaling Pathways Regulate the Motor Output of Cochlear Outer Hair Cells J. Neurosci., August 15, 2000; 20(16): 5940 - 5948. [Abstract] [Full Text] [PDF]

				J. S. Oghalai, H. Zhao, J. W. Kutz, and W. E. Brownell Voltage- and Tension-Dependent Lipid Mobility in the Outer Hair Cell Plasma Membrane Science, January 28, 2000; 287(5453): 658 - 661. [Abstract] [Full Text]

				W. Marcotti and C. J Kros Developmental expression of the potassium current IK,n contributes to maturation of mouse outer hair cells J. Physiol., November 1, 1999; 520(3): 653 - 660. [Abstract] [Full Text] [PDF]

				D. Oliver and B. Fakler Expression density and functional characteristics of the outer hair cell motor protein are regulated during postnatal development in rat J. Physiol., September 15, 1999; 519(3): 791 - 800. [Abstract] [Full Text] [PDF]

				D. Z. Z. He and P. Dallos Somatic stiffness of cochlear outer hair cells is voltage-dependent PNAS, July 6, 1999; 96(14): 8223 - 8228. [Abstract] [Full Text] [PDF]

				M. Adachi and K. H. Iwasa Electrically driven motor in the outer hair cell: Effect of a mechanical constraint PNAS, June 22, 1999; 96(13): 7244 - 7249. [Abstract] [Full Text] [PDF]

				G. Frank, W. Hemmert, and A. W. Gummer Limiting dynamics of high-frequency electromechanical transduction of outer hair cells PNAS, April 13, 1999; 96(8): 4420 - 4425. [Abstract] [Full Text] [PDF]

				G. I. Frolenkov, M. Atzori, F. Kalinec, F. Mammano, and B. Kachar The Membrane-based Mechanism of Cell Motility in Cochlear Outer Hair Cells Mol. Biol. Cell, August 1, 1998; 9(8): 1961 - 1968. [Full Text]

				J Santos-Sacchi, S Kakehata, and S Takahashi Effects of membrane potential on the voltage dependence of motility-related charge in outer hair cells of the guinea-pig J. Physiol., July 1, 1998; 510(1): 225 - 235. [Abstract] [Full Text] [PDF]

				J. S. Oghalai, A. A. Patel, T. Nakagawa, and W. E. Brownell Fluorescence-Imaged Microdeformation of the Outer Hair Cell Lateral Wall J. Neurosci., January 1, 1998; 18(1): 48 - 58. [Abstract] [Full Text] [PDF]

				P. Dallos, D. Z. Z. He, X. Lin, I. Sziklai, S. Mehta, and B. N. Evans Acetylcholine, Outer Hair Cell Electromotility, and the Cochlear Amplifier J. Neurosci., March 15, 1997; 17(6): 2212 - 2226. [Abstract] [Full Text] [PDF]

				S. Kakehata and J. Santos-Sacchi Effects of Salicylate and Lanthanides on Outer Hair Cell Motility and Associated Gating Charge J. Neurosci., August 15, 1996; 16(16): 4881 - 4889. [Abstract] [Full Text] [PDF]

				P Dallos and B. Evans High-frequency motility of outer hair cells and the cochlear amplifier Science, March 31, 1995; 267(5206): 2006 - 2009. [Abstract] [PDF]

				I. A. Belyantseva, H. J. Adler, R. Curi, G. I. Frolenkov, and B. Kachar Expression and Localization of Prestin and the Sugar Transporter GLUT-5 during Development of Electromotility in Cochlear Outer Hair Cells J. Neurosci., December 15, 2000; 20(24): RC116 - RC116. [Abstract] [Full Text] [PDF]

				N. Morimoto, R. M. Raphael, A. Nygren, and W. E. Brownell Excess plasma membrane and effects of ionic amphipaths on mechanics of outer hair cell lateral wall Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1076 - C1086. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help