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Journal of Neuroscience, Vol 11, 3565-3572, Copyright © 1991 by Society for Neuroscience
Single odor-sensitive channels in olfactory receptor neurons are also gated by cyclic nucleotides
S Firestein, F Zufall and GM Shepherd
Section of Neurobiology, Yale University Medical School, New Haven, Connecticut 06510.
Olfactory transduction is thought to occur by processes that are mainly
restricted to the specialized cilia emanating from the distal end of the
receptor neuron's single dendrite. The involvement of a cAMP-based second
messenger system seems likely, and a cyclic nucleotide-sensitive current
has been recorded in patches of membrane from the cilia. However, the small
diameter of the cilia and the high density of channels within the membrane
limit the application of the patch recording technique in the cilia. We
have found that the cAMP-sensitive channels also exist at a much lower
density within the far more accessible dendritic membrane. Recording from
on-cell patches, we have observed single-channel activity in response to
extracellularly applied odor substances. The channels have a single-channel
conductance of 40 pS and a reversal potential near 0 mV. These same
channels are activated by treatments that elevate intracellular cyclic
nucleotide concentrations. The results provide a direct demonstration that
the cyclic nucleotide-gated channel is the conductance pathway for the
odor- elicited current.
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