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Journal of Neuroscience, Vol 11, 2075-2086, Copyright © 1991 by Society for Neuroscience
Immunohistochemical localization of an inositol 1,4,5-trisphosphate receptor, P400, in neural tissue: studies in developing and adult mouse brain
S Nakanishi, N Maeda and K Mikoshiba
Division of Regulation of Macromolecular Function, Osaka University, Suita, Japan.
The immunohistochemical localization of P400/inositol 1,4,5- trisphosphate
(InsP3) receptor protein was studied in developing and adult mouse brain by
using monoclonal antibodies. The developmental expression pattern of
P400/InsP3 receptor protein differed among different classes of neurons. It
was first detected in the somata of immature Purkinje cells at embryonic
day 17, in the ventrolateral region of the posterior vermis in the
cerebellum. Axonal immunoreactivity within the cerebellar nuclei was first
present at postnatal day 3. Neurons in the retrosplenial cortex, the
anterior olfactory nucleus, and the CA1 region of the hippocampus expressed
immunoreactivity earlier than other regions of the brain. In the adult
brain, not only the Purkinje cell but also many other types of cells in
many areas of the brain expressed P400/InsP3 receptor, though to a lesser
extent. These included the neurons in the striatum, globus pallidus,
nucleus accumbens septi, anterior olfactory nucleus, olfactory tubercle,
precommissural hippocampus, hippocampus, substantia nigra, cerebral cortex,
pons, and certain hypothalamic nuclei. Forebrain cortical regions that
receive afferents from the olfactory bulb, such as the anterior olfactory
nucleus, olfactory tubercle, prepiriform cortex, entorhinal cortex, and
amygdala, exhibited distinct immunoreactivity, while olfactory bulb was
almost devoid of staining. Immunoreactivity in the axonal pathways was also
found in the limbic- hypothalamic pathways, strionigral projection, and
part of the corpus callosum. Results of Western blot analysis and 3H-InsP3
binding assay were consistent with the qualitative regional differences of
immunoreactivity demonstrated by immunohistochemical study. The location of
InsP3 receptor in the brain correlates well with the InsP3 binding sites
demonstrated by an autoradiographic study.
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